Purification and Characterization of Catalase from Marine Bacterium Acinetobacter sp. YS0810
Joint Authors
Wang, Wei
Sun, Mi
Hao, Jianhua
Zhu, Xianglin
Fu, Xinhua
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-06-18
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
The catalase from marine bacterium Acinetobacter sp.
YS0810 (YS0810CAT) was purified and characterized.
Consecutive steps were used to achieve the purified enzyme as follows: ethanol precipitation, DEAE Sepharose ion exchange, Superdex 200 gel filtration, and Resource Q ion exchange.
The active enzyme consisted of four identical subunits of 57.256 kDa.
It showed a Soret peak at 405 nm, indicating the presence of iron protoporphyrin IX.
The catalase was not apparently reduced by sodium dithionite but was inhibited by 3-amino-1,2,4-triazole, hydroxylamine hydrochloride, and sodium azide.
Peroxidase-like activity was not found with the substrate o-phenylenediamine.
So the catalase was determined to be a monofunctional catalase.
N-terminal amino acid of the catalase analysis gave the sequence SQDPKKCPVTHLTTE, which showed high degree of homology with those of known catalases from bacteria.
The analysis of amino acid sequence of the purified catalase by matrix-assisted laser desorption ionization time-of-flight mass spectrometry showed that it was a new catalase, in spite of its high homology with those of known catalases from other bacteria.
The catalase showed high alkali stability and thermostability.
American Psychological Association (APA)
Fu, Xinhua& Wang, Wei& Hao, Jianhua& Zhu, Xianglin& Sun, Mi. 2014. Purification and Characterization of Catalase from Marine Bacterium Acinetobacter sp. YS0810. BioMed Research International،Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-469904
Modern Language Association (MLA)
Fu, Xinhua…[et al.]. Purification and Characterization of Catalase from Marine Bacterium Acinetobacter sp. YS0810. BioMed Research International No. 2014 (2014), pp.1-7.
https://search.emarefa.net/detail/BIM-469904
American Medical Association (AMA)
Fu, Xinhua& Wang, Wei& Hao, Jianhua& Zhu, Xianglin& Sun, Mi. Purification and Characterization of Catalase from Marine Bacterium Acinetobacter sp. YS0810. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-469904
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-469904