Screening for Residual Disease in Pediatric Burkitt Lymphoma Using Consensus Primer Pools
Joint Authors
Kusao, Ian
Troelstrup, David
Shiramizu, Bruce
Agsalda, Melissa
Source
Issue
Vol. 2009, Issue 2009 (31 Dec. 2009), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2009-04-15
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Assessing molecular persistent or minimal residual disease (PD/MRD) in childhood Burkitt lymphoma (BL) is challenging because access to original tumor is usually needed to design patient-specific primers (PSPs).
Because BL is characterized by rearranged immunoglobulin heavy chain (IgVH) genes, IgVH primer pools from IgVH1–IgVH7 regions were tested to detect PD/MRD, thus eliminating the need for original tumor.
The focus of the current study was to assess the feasibility of using IgVH primer pools to detect disease in clinical specimens.
Fourteen children diagnosed with B-NHL had follow-up repository specimens available to assess PD/MRD.
Of the 14 patients, 12 were PD/MRD negative after 2 months of therapy and remained in remission at the end of therapy; 2/14 patients were PD/MRD positive at 2-3 months and later relapsed.
PSP-based assays from these 14 patients showed 100% concordance with the current assay.
This feasibility study warrants further investigation to assess PD/MRD using IgVH primer pools, which could have clinical significance as a real-time assessment tool to monitor pediatric BL and possibly other B-cell non-Hodgkin lymphoma therapy.
American Psychological Association (APA)
Agsalda, Melissa& Kusao, Ian& Troelstrup, David& Shiramizu, Bruce. 2009. Screening for Residual Disease in Pediatric Burkitt Lymphoma Using Consensus Primer Pools. Advances in Hematology،Vol. 2009, no. 2009, pp.1-7.
https://search.emarefa.net/detail/BIM-470036
Modern Language Association (MLA)
Agsalda, Melissa…[et al.]. Screening for Residual Disease in Pediatric Burkitt Lymphoma Using Consensus Primer Pools. Advances in Hematology No. 2009 (2009), pp.1-7.
https://search.emarefa.net/detail/BIM-470036
American Medical Association (AMA)
Agsalda, Melissa& Kusao, Ian& Troelstrup, David& Shiramizu, Bruce. Screening for Residual Disease in Pediatric Burkitt Lymphoma Using Consensus Primer Pools. Advances in Hematology. 2009. Vol. 2009, no. 2009, pp.1-7.
https://search.emarefa.net/detail/BIM-470036
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-470036