SSR Locator : Tool for Simple Sequence Repeat Discovery Integrated with Primer Design and PCR Simulation
Joint Authors
Souza, Velci Queiroz de
Carvalho, Fernando Irajá Félix de
Costa de Oliveira, Antonio
Kopp, Mauricio Marini
Palmieri, Dario Abel
Maia, Luciano Carlos da
Source
International Journal of Plant Genomics
Issue
Vol. 2008, Issue 2008 (31 Dec. 2008), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2008-07-27
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Microsatellites or SSRs (simple sequence repeats) are ubiquitous short tandem duplications occurring in eukaryotic organisms.
These sequences are among the best marker technologies applied in plant genetics and breeding.
The abundant genomic, BAC, and EST sequences available in databases allow the survey regarding presence and location of SSR loci.
Additional information concerning primer sequences is also the target of plant geneticists and breeders.
In this paper, we describe a utility that integrates SSR searches, frequency of occurrence of motifs and arrangements, primer design, and PCR simulation against other databases.
This simulation allows the performance of global alignments and identity and homology searches between different amplified sequences, that is, amplicons.
In order to validate the tool functions, SSR discovery searches were performed in a database containing 28 469 nonredundant rice cDNA sequences.
American Psychological Association (APA)
Maia, Luciano Carlos da& Palmieri, Dario Abel& Souza, Velci Queiroz de& Kopp, Mauricio Marini& Carvalho, Fernando Irajá Félix de& Costa de Oliveira, Antonio. 2008. SSR Locator : Tool for Simple Sequence Repeat Discovery Integrated with Primer Design and PCR Simulation. International Journal of Plant Genomics،Vol. 2008, no. 2008, pp.1-9.
https://search.emarefa.net/detail/BIM-470090
Modern Language Association (MLA)
Maia, Luciano Carlos da…[et al.]. SSR Locator : Tool for Simple Sequence Repeat Discovery Integrated with Primer Design and PCR Simulation. International Journal of Plant Genomics No. 2008 (2008), pp.1-9.
https://search.emarefa.net/detail/BIM-470090
American Medical Association (AMA)
Maia, Luciano Carlos da& Palmieri, Dario Abel& Souza, Velci Queiroz de& Kopp, Mauricio Marini& Carvalho, Fernando Irajá Félix de& Costa de Oliveira, Antonio. SSR Locator : Tool for Simple Sequence Repeat Discovery Integrated with Primer Design and PCR Simulation. International Journal of Plant Genomics. 2008. Vol. 2008, no. 2008, pp.1-9.
https://search.emarefa.net/detail/BIM-470090
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-470090