Gene Expression of Adhesion Molecules in Endothelial Cells from Patients with Peripheral Arterial Disease Is Reduced after Surgical Revascularization and Pharmacological Treatment
Joint Authors
Schembri, Laura
Guasti, Luigina
Cosentino, Marco
Castiglioni, Luana
Molteni, Elisabetta
Piffaretti, Gabriele
Marino, Franca
Castelli, Patrizio
Tozzi, Matteo
Source
International Journal of Vascular Medicine
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-02-27
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Atherosclerosis is an inflammatory disease characterized by immunological activity, in which endothelial dysfunction represents an early event leading to subsequent inflammatory vascular damage.
We investigated gene expression of the adhesion molecules (AMs) ICAM-1, VCAM-1, and β1-integrin in endothelial cells (ECs) isolated from venous blood (circulating EC, cEC) and purified from femoral plaques (pEC) obtained from 9 patients with peripheral artery disease (PAD) submitted to femoral artery thrombendarterectomy (FEA).
In addition, in peripheral blood mononuclear cells (PBMCs) of the same subjects, we investigated gene expression of IFN-γ, IL-4, TGF-β, and IL-10.
Patients were longitudinally evaluated 1 month before surgery, when statin treatment was established, at the time of surgery, and after 2 and 5 months.
All AM mRNA levels, measured by means of real-time PCR, in cEC diminished during the study, up to 41–50% of initial levels at followup.
AM mRNA expression was significantly higher in pEC than in cEC.
During the study, in PBMCs, TGF-β and IL-10 mRNA levels remained unchanged while IFN-γ and IL-4 levels increased; however, the ratio IFN-γ/IL-4 showed no significant modification.
In PAD patients, FEA and statin treatment induce a profound reduction of AM expression in cEC and affect cytokine mRNA expression in PBMCs.
American Psychological Association (APA)
Marino, Franca& Guasti, Luigina& Tozzi, Matteo& Schembri, Laura& Castiglioni, Luana& Molteni, Elisabetta…[et al.]. 2013. Gene Expression of Adhesion Molecules in Endothelial Cells from Patients with Peripheral Arterial Disease Is Reduced after Surgical Revascularization and Pharmacological Treatment. International Journal of Vascular Medicine،Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-470102
Modern Language Association (MLA)
Marino, Franca…[et al.]. Gene Expression of Adhesion Molecules in Endothelial Cells from Patients with Peripheral Arterial Disease Is Reduced after Surgical Revascularization and Pharmacological Treatment. International Journal of Vascular Medicine No. 2013 (2013), pp.1-8.
https://search.emarefa.net/detail/BIM-470102
American Medical Association (AMA)
Marino, Franca& Guasti, Luigina& Tozzi, Matteo& Schembri, Laura& Castiglioni, Luana& Molteni, Elisabetta…[et al.]. Gene Expression of Adhesion Molecules in Endothelial Cells from Patients with Peripheral Arterial Disease Is Reduced after Surgical Revascularization and Pharmacological Treatment. International Journal of Vascular Medicine. 2013. Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-470102
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-470102