Dynamics of Spreading and Alignment of Cells Cultured In Vitro on a Grooved Polymer Surface
Joint Authors
Romanin, Christoph
Peterbauer, Thomas
Siegel, Jakub
Fahrner, Marc
Heitz, Johannes
Hering, Steffen
Yakunin, Sergii
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-08-09
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Engineering Sciences and Information Technology
Chemistry
Civil Engineering
Abstract EN
We used mechanically embossed polyester films to analyze the dynamics of cell alignment and cell-specific factors modulating the response of Chinese hamster ovary (CHO) cells and of a rat myogenic cell line to the surface topography.
The films used had grooves with a periodicity of approximately 750 nm and a depth of 150 nm.
Both cell lines responded to the topographical feature.
On unpatterned control areas, cells of both lines showed a random distribution with orientation angles close to 45∘.
Both cell types exhibited an elongated morphology on the patterned surface.
CHO cells typically showed bipolar spreading.
Their contact area increased almost exclusively along the groove direction.
Likewise, freshly seeded rat myoblasts displayed protrusions emerging in parallel with the grooves.
However, myoblasts frequently had more than two sites with plasma protrusions pulling the cells along different grooves.
They could also develop lamellipodia expanding without a preferred direction and long filopodia.
American Psychological Association (APA)
Peterbauer, Thomas& Yakunin, Sergii& Siegel, Jakub& Hering, Steffen& Fahrner, Marc& Romanin, Christoph…[et al.]. 2010. Dynamics of Spreading and Alignment of Cells Cultured In Vitro on a Grooved Polymer Surface. Journal of Nanomaterials،Vol. 2011, no. 2011, pp.1-10.
https://search.emarefa.net/detail/BIM-470139
Modern Language Association (MLA)
Peterbauer, Thomas…[et al.]. Dynamics of Spreading and Alignment of Cells Cultured In Vitro on a Grooved Polymer Surface. Journal of Nanomaterials No. 2011 (2011), pp.1-10.
https://search.emarefa.net/detail/BIM-470139
American Medical Association (AMA)
Peterbauer, Thomas& Yakunin, Sergii& Siegel, Jakub& Hering, Steffen& Fahrner, Marc& Romanin, Christoph…[et al.]. Dynamics of Spreading and Alignment of Cells Cultured In Vitro on a Grooved Polymer Surface. Journal of Nanomaterials. 2010. Vol. 2011, no. 2011, pp.1-10.
https://search.emarefa.net/detail/BIM-470139
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-470139