Saikosaponin a Enhances Transient Inactivating Potassium Current in Rat Hippocampal CA1 Neurons
Joint Authors
Xing, Jun Ling
Yu, Lin
Li, Chang Zheng
Xie, Wei
Zhao, Yun Yan
Duan, Jian Hong
Yu, Yun Hong
Du, Yong Ping
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-02-21
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Saikosaponin a (SSa), a main constituent of the Chinese herb Bupleurum chinense DC., has been demonstrated to have antiepileptic activity.
Recent studies have shown that SSa could inhibit NMDA receptor current and persistent sodium current.
However, the effects of SSa on potassium (K+) currents remain unclear.
In this study, we tested the effect of SSa on 4AP-induced epileptiform discharges and K+ currents in CA1 neurons of rat hippocampal slices.
We found that SSa significantly inhibited epileptiform discharges frequency and duration in hippocampal CA1 neurons in the 4AP seizure model in a dose-dependent manner with an IC50 of 0.7 μM.
SSa effectively increased the amplitude of ITotal and IA, significantly negative-shifted the activation curve, and positive-shifted steady-state curve of IA.
However, SSa induced no significant changes in the amplitude and activation curve of IK.
In addition, SSa significantly increased the amplitude of 4AP-sensitive K+ current, while there was no significant change in the amplitude of TEA-sensitive K+ current.
Together, our data indicate that SSa inhibits epileptiform discharges induced by 4AP in a dose-dependent manner and that SSa exerts selectively enhancing effects on IA.
These increases in IA may contribute to the anticonvulsant mechanisms of SSa.
American Psychological Association (APA)
Xie, Wei& Yu, Yun Hong& Du, Yong Ping& Zhao, Yun Yan& Li, Chang Zheng& Yu, Lin…[et al.]. 2013. Saikosaponin a Enhances Transient Inactivating Potassium Current in Rat Hippocampal CA1 Neurons. Evidence-Based Complementary and Alternative Medicine،Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-470142
Modern Language Association (MLA)
Xie, Wei…[et al.]. Saikosaponin a Enhances Transient Inactivating Potassium Current in Rat Hippocampal CA1 Neurons. Evidence-Based Complementary and Alternative Medicine No. 2013 (2013), pp.1-10.
https://search.emarefa.net/detail/BIM-470142
American Medical Association (AMA)
Xie, Wei& Yu, Yun Hong& Du, Yong Ping& Zhao, Yun Yan& Li, Chang Zheng& Yu, Lin…[et al.]. Saikosaponin a Enhances Transient Inactivating Potassium Current in Rat Hippocampal CA1 Neurons. Evidence-Based Complementary and Alternative Medicine. 2013. Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-470142
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-470142