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Protective Effect of Scutellaria litwinowii Extract on SerumGlucose-Deprived Cultured PC12 Cells and Determining the Role of Reactive Oxygen Species
Joint Authors
Mousavi, Seyed Hadi
Tayarani-Najaran, Zahra
Zare, Aryo
Afsharzadeh, Maryam
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-07-19
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Pharmacy, Health & Medical Sciences
Medicine
Abstract EN
Considering the wide, positive reporting of the role of reactive oxygen species in ischemic brain injury, searching for antioxidant drugs within herbal remedies is logical.
In this study, the protective effects of Scutellaria litwinowii Bornm.
& Sint.
on cell viability and reactive oxygen species production in cultured PC12 cells were investigated under serum/glucose-deprivation-induced cell death.
After cells were seeded overnight, they were then deprived of serum/glucose for 24 h.
Cells were treated with different concentrations of S.
litwinowii extract (7.75–250 μg/mL).
Cell viability was quantitated by MTT assay, and intracellular reactive oxygen species production was measured by flow cytometry.
Serum/glucose-deprivation induced significant cell death after 24 h (P < 0.001).
Treatment with S.
litwinowii (7.75–250 μg/mL) reduced serum/glucose deprivation-induced cytotoxicity in PC12 cells after 24 h.
A significant increase in intracellular reactive oxygen species production was seen following serum/glucose deprivation (P < 0.001).
S.
litwinowii (62 and 125 μg/mL, P < 0.01) treatment reversed the increased reactive oxygen species production following ischemic insult.
This demonstrates that S.
litwinowii extract protects PC12 cells against serum/glucose-deprivation-induced cell death by antioxidant mechanisms, which indicates the potential therapeutic application of S.
litwinowii in managing cerebral ischemic and neurodegenerative disorders.
American Psychological Association (APA)
Afsharzadeh, Maryam& Tayarani-Najaran, Zahra& Zare, Aryo& Mousavi, Seyed Hadi. 2012. Protective Effect of Scutellaria litwinowii Extract on SerumGlucose-Deprived Cultured PC12 Cells and Determining the Role of Reactive Oxygen Species. Journal of Toxicology،Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-470161
Modern Language Association (MLA)
Afsharzadeh, Maryam…[et al.]. Protective Effect of Scutellaria litwinowii Extract on SerumGlucose-Deprived Cultured PC12 Cells and Determining the Role of Reactive Oxygen Species. Journal of Toxicology No. 2012 (2012), pp.1-6.
https://search.emarefa.net/detail/BIM-470161
American Medical Association (AMA)
Afsharzadeh, Maryam& Tayarani-Najaran, Zahra& Zare, Aryo& Mousavi, Seyed Hadi. Protective Effect of Scutellaria litwinowii Extract on SerumGlucose-Deprived Cultured PC12 Cells and Determining the Role of Reactive Oxygen Species. Journal of Toxicology. 2012. Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-470161
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-470161