In Vitro and Ex Vivo Evaluation of Smart Infra-Red Fluorescent Caspase-3 Probes for Molecular Imaging of Cardiovascular Apoptosis
Joint Authors
Delest, Bruno
Massonneau, Marc
Brakenhielm, Ebba
Debunne, Manuelle
Renard, Pierre-Yves
Thuillez, Christian
Ligeret, Heidi
Richard, Vincent
Noack, Pauline
Henry, Jean-Paul
Romieu, Anthony
Lallemand, Françoise
Portal, Christophe
Source
International Journal of Molecular Imaging
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-05-05
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Purpose.
The aim of this paper is to develop new optical bioprobes for the imaging of apoptosis.
Procedure.
We developed quenched near-infrared probes which become fluorescent upon cleavage by caspase-3, the key regulatory enzyme of apoptosis.
Results.
Probes were shown to be selectively cleaved by recombinant caspase-3.
Apoptosis of cultured endothelial cells was associated with an increased fluorescent signal for the cleaved probes, which colocalized with caspase-3 and was reduced by the addition of a caspase-3 inhibitor.
Flow cytometry demonstrated a similar profile between the cleaved probes and annexin V.
Ex vivo experiments showed that sections of hearts obtained from mice treated with the proapoptotic drug doxorubicin displayed an increase in the fluorescent signal for the cleaved probes, which was reduced by a caspase-3 inhibitor.
Conclusion.
We demonstrated the capacity of these novel probes to detect apoptosis by optical imaging in vitro and ex vivo.
American Psychological Association (APA)
Debunne, Manuelle& Portal, Christophe& Delest, Bruno& Brakenhielm, Ebba& Lallemand, Françoise& Henry, Jean-Paul…[et al.]. 2011. In Vitro and Ex Vivo Evaluation of Smart Infra-Red Fluorescent Caspase-3 Probes for Molecular Imaging of Cardiovascular Apoptosis. International Journal of Molecular Imaging،Vol. 2011, no. 2011, pp.1-13.
https://search.emarefa.net/detail/BIM-470162
Modern Language Association (MLA)
Debunne, Manuelle…[et al.]. In Vitro and Ex Vivo Evaluation of Smart Infra-Red Fluorescent Caspase-3 Probes for Molecular Imaging of Cardiovascular Apoptosis. International Journal of Molecular Imaging No. 2011 (2011), pp.1-13.
https://search.emarefa.net/detail/BIM-470162
American Medical Association (AMA)
Debunne, Manuelle& Portal, Christophe& Delest, Bruno& Brakenhielm, Ebba& Lallemand, Françoise& Henry, Jean-Paul…[et al.]. In Vitro and Ex Vivo Evaluation of Smart Infra-Red Fluorescent Caspase-3 Probes for Molecular Imaging of Cardiovascular Apoptosis. International Journal of Molecular Imaging. 2011. Vol. 2011, no. 2011, pp.1-13.
https://search.emarefa.net/detail/BIM-470162
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-470162