Inhibitory Effects of Hwangryunhaedok-Tang in 3T3-L1 Adipogenesis by Regulation of RafMEK1ERK12 Pathway and PDK1Akt Phosphorylation
Joint Authors
Ma, Jin Yeul
Lee, Kwang Jin
Kwak, Dong Hoon
Lee, Ji-Hye
Kim, Dong-Gun
Kim, Taesoo
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-05-23
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Hwangryunhaedok-tang (HRT) has been long used as traditional medicine in Asia.
However, inhibitory role of HRT is unclear in early stage of 3T3-L1 adipocyte differentiation related to signaling.
In the present study, we investigated the inhibitory effects of HRT on upstream signaling of peroxisome proliferation-activity receptor-γ (PPAR-γ) and CCAAT/enhancer binding protein-β (C/EBP-β) expression in differentiation of 3T3-L1 preadipocytes.
We found that HRT significantly inhibited the adipocyte differentiation by downregulating several adipocyte-specific transcription factors including PPAR-γ, C/EBP-α, and C/EBP-β in 3T3-L1 preadipocytes.
Furthermore, we observed that HRT markedly inhibited the differentiation media-mediated phosphorylation of Raf/extracellular mitogen-activated protein kinase 1 (MEK1)/signal-regulated protein kinase 1/2 (ERK1/2) and phosphorylation of phosphoinositide-dependent kinase 1 (PDK1)/Akt.
These results indicate that anti-adipogenesis mechanism involves the downregulation of the major transcription factors of adipogenesis including PPAR-γ and C/EBP-α through inhibition of Raf/MEK1/ERK1/2 phosphorylation and PDK1/Akt phosphorylation by HRT.
Furthermore, high performance liquid chromatography (HPLC) analysis showed HRT contains active antiobesity constituents such as palmatine, berberine, geniposide, baicalin, baicalein, and wogonin.
Taken together, this study suggested that anti-adipogenesis effects of HRT were accounted by downregulation of Raf/MEK1/ERK1/2 pathway and PDK1/Akt pathway during 3T3-L1 adipocyte differentiation.
American Psychological Association (APA)
Kwak, Dong Hoon& Lee, Ji-Hye& Kim, Dong-Gun& Kim, Taesoo& Lee, Kwang Jin& Ma, Jin Yeul. 2013. Inhibitory Effects of Hwangryunhaedok-Tang in 3T3-L1 Adipogenesis by Regulation of RafMEK1ERK12 Pathway and PDK1Akt Phosphorylation. Evidence-Based Complementary and Alternative Medicine،Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-470218
Modern Language Association (MLA)
Kwak, Dong Hoon…[et al.]. Inhibitory Effects of Hwangryunhaedok-Tang in 3T3-L1 Adipogenesis by Regulation of RafMEK1ERK12 Pathway and PDK1Akt Phosphorylation. Evidence-Based Complementary and Alternative Medicine No. 2013 (2013), pp.1-12.
https://search.emarefa.net/detail/BIM-470218
American Medical Association (AMA)
Kwak, Dong Hoon& Lee, Ji-Hye& Kim, Dong-Gun& Kim, Taesoo& Lee, Kwang Jin& Ma, Jin Yeul. Inhibitory Effects of Hwangryunhaedok-Tang in 3T3-L1 Adipogenesis by Regulation of RafMEK1ERK12 Pathway and PDK1Akt Phosphorylation. Evidence-Based Complementary and Alternative Medicine. 2013. Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-470218
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-470218