50 Hz Electromagnetic Field Produced Changes in FTIR Spectroscopy Associated with Mitochondrial Transmembrane Potential Reduction in Neuronal-Like SH-SY5Y Cells

Joint Authors

Magazù, Salvatore
Ientile, Riccardo
Caccamo, Daniela
Condello, Salvatore
Currò, Monica
Ferlazzo, Nadia
Vecchio, Mercurio
Calabrò, Emanuele

Source

Oxidative Medicine and Cellular Longevity

Issue

Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-8, 8 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2013-07-16

Country of Publication

Egypt

No. of Pages

8

Main Subjects

Natural & Life Sciences (Multidisciplinary)
Biology

Abstract EN

SH-SY5Y neuroblastoma cells were used as an experimental model to study the effects of 50 Hz electromagnetic field, in the range from 50 µT to 1.4 mT.

Fourier transform infrared spectroscopy analysis evidenced a reduction in intensity of the amide A band and a slight increase of vibration bands at 2921 cm−1 and 2853 cm−1 corresponding to methylene groups.

A further increase of the magnetic field intensity of exposure up to 0.8 mT and 1.4 mT produced a clear increase in intensity of CH2 vibration bands.

Moreover, it has been observed some alterations in the amide I region, such as a shifted peak of the amide I band to a smaller wavenumber, probably due to protein conformational changes.

These results suggested that exposure to extremely low electromagnetic fields influenced lipid components of cellular membrane and the N–H in-plane bending and C–N stretching vibrations of peptide linkages, modifying the secondary structures of α-helix and β-sheet contents and producing unfolding process in cell membrane proteins.

The observed changes after exposure to 50 Hz electromagnetic field higher than 0.8 mT were associated with a significant reduction of cell viability and reduced mitochondrial transmembrane potential.

American Psychological Association (APA)

Calabrò, Emanuele& Condello, Salvatore& Currò, Monica& Ferlazzo, Nadia& Vecchio, Mercurio& Caccamo, Daniela…[et al.]. 2013. 50 Hz Electromagnetic Field Produced Changes in FTIR Spectroscopy Associated with Mitochondrial Transmembrane Potential Reduction in Neuronal-Like SH-SY5Y Cells. Oxidative Medicine and Cellular Longevity،Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-470265

Modern Language Association (MLA)

Calabrò, Emanuele…[et al.]. 50 Hz Electromagnetic Field Produced Changes in FTIR Spectroscopy Associated with Mitochondrial Transmembrane Potential Reduction in Neuronal-Like SH-SY5Y Cells. Oxidative Medicine and Cellular Longevity No. 2013 (2013), pp.1-8.
https://search.emarefa.net/detail/BIM-470265

American Medical Association (AMA)

Calabrò, Emanuele& Condello, Salvatore& Currò, Monica& Ferlazzo, Nadia& Vecchio, Mercurio& Caccamo, Daniela…[et al.]. 50 Hz Electromagnetic Field Produced Changes in FTIR Spectroscopy Associated with Mitochondrial Transmembrane Potential Reduction in Neuronal-Like SH-SY5Y Cells. Oxidative Medicine and Cellular Longevity. 2013. Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-470265

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-470265