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50 Hz Electromagnetic Field Produced Changes in FTIR Spectroscopy Associated with Mitochondrial Transmembrane Potential Reduction in Neuronal-Like SH-SY5Y Cells
Joint Authors
Magazù, Salvatore
Ientile, Riccardo
Caccamo, Daniela
Condello, Salvatore
Currò, Monica
Ferlazzo, Nadia
Vecchio, Mercurio
Calabrò, Emanuele
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-07-16
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Biology
Abstract EN
SH-SY5Y neuroblastoma cells were used as an experimental model to study the effects of 50 Hz electromagnetic field, in the range from 50 µT to 1.4 mT.
Fourier transform infrared spectroscopy analysis evidenced a reduction in intensity of the amide A band and a slight increase of vibration bands at 2921 cm−1 and 2853 cm−1 corresponding to methylene groups.
A further increase of the magnetic field intensity of exposure up to 0.8 mT and 1.4 mT produced a clear increase in intensity of CH2 vibration bands.
Moreover, it has been observed some alterations in the amide I region, such as a shifted peak of the amide I band to a smaller wavenumber, probably due to protein conformational changes.
These results suggested that exposure to extremely low electromagnetic fields influenced lipid components of cellular membrane and the N–H in-plane bending and C–N stretching vibrations of peptide linkages, modifying the secondary structures of α-helix and β-sheet contents and producing unfolding process in cell membrane proteins.
The observed changes after exposure to 50 Hz electromagnetic field higher than 0.8 mT were associated with a significant reduction of cell viability and reduced mitochondrial transmembrane potential.
American Psychological Association (APA)
Calabrò, Emanuele& Condello, Salvatore& Currò, Monica& Ferlazzo, Nadia& Vecchio, Mercurio& Caccamo, Daniela…[et al.]. 2013. 50 Hz Electromagnetic Field Produced Changes in FTIR Spectroscopy Associated with Mitochondrial Transmembrane Potential Reduction in Neuronal-Like SH-SY5Y Cells. Oxidative Medicine and Cellular Longevity،Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-470265
Modern Language Association (MLA)
Calabrò, Emanuele…[et al.]. 50 Hz Electromagnetic Field Produced Changes in FTIR Spectroscopy Associated with Mitochondrial Transmembrane Potential Reduction in Neuronal-Like SH-SY5Y Cells. Oxidative Medicine and Cellular Longevity No. 2013 (2013), pp.1-8.
https://search.emarefa.net/detail/BIM-470265
American Medical Association (AMA)
Calabrò, Emanuele& Condello, Salvatore& Currò, Monica& Ferlazzo, Nadia& Vecchio, Mercurio& Caccamo, Daniela…[et al.]. 50 Hz Electromagnetic Field Produced Changes in FTIR Spectroscopy Associated with Mitochondrial Transmembrane Potential Reduction in Neuronal-Like SH-SY5Y Cells. Oxidative Medicine and Cellular Longevity. 2013. Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-470265
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-470265