![](/images/graphics-bg.png)
Glutaminase Immunoreactivity and Enzyme Activity Is Increased in the Rat Dorsal Root Ganglion Following Peripheral Inflammation
Joint Authors
Balbás, John C.
Kriebel, Richard M.
Miller, Kenneth E.
Benton, Richard L.
Lam, Travis S.
Schechter, Ruben
Edwards, Kristin M.
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-12-20
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Following inflammation, primary sensory neurons in the dorsal root ganglion (DRG) alter the production of several proteins.
Most DRG neurons are glutamatergic, using glutaminase as the enzyme for glutamate production, but little is known about glutaminase following inflammation.
In the present study, adjuvant-induced arthritis (AIA) was produced in rats with complete Freund's adjuvant into the hindpaw.
At 7 days of AIA, DRG were examined with glutaminase immunohistochemistry, Western blot immunoreactivity, and enzyme activity.
Image analysis revealed that glutaminase was elevated most in small-sized neurons (21%) (P < 0.05).
Western blot analysis revealed a 19% increase (P < 0.05) in total glutaminase and 21% in mitochondrial glutaminase (P < 0.05).
Glutaminase enzyme activity was elevated 29% (P < 0.001) from 2.20 to 2.83 moles/kg/hr.
Elevated glutaminase in primary sensory neurons could lead to increased glutamate production in spinal primary afferent terminals contributing to central sensitization or in the peripheral process contributing to peripheral sensitization.
American Psychological Association (APA)
Miller, Kenneth E.& Balbás, John C.& Benton, Richard L.& Lam, Travis S.& Edwards, Kristin M.& Kriebel, Richard M.…[et al.]. 2011. Glutaminase Immunoreactivity and Enzyme Activity Is Increased in the Rat Dorsal Root Ganglion Following Peripheral Inflammation. Pain Research and Treatment،Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-470278
Modern Language Association (MLA)
Miller, Kenneth E.…[et al.]. Glutaminase Immunoreactivity and Enzyme Activity Is Increased in the Rat Dorsal Root Ganglion Following Peripheral Inflammation. Pain Research and Treatment No. 2012 (2012), pp.1-9.
https://search.emarefa.net/detail/BIM-470278
American Medical Association (AMA)
Miller, Kenneth E.& Balbás, John C.& Benton, Richard L.& Lam, Travis S.& Edwards, Kristin M.& Kriebel, Richard M.…[et al.]. Glutaminase Immunoreactivity and Enzyme Activity Is Increased in the Rat Dorsal Root Ganglion Following Peripheral Inflammation. Pain Research and Treatment. 2011. Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-470278
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-470278