Fibrinogen Alpha Chain Precursor and Apolipoprotein A-I in Urine as Biomarkers for Noninvasive Diagnosis of Calcium Oxalate Nephrolithiasis : A Proteomics Study
Joint Authors
Wang, Guang-Chun
Zhu, Wei
Peng, Bo
Che, Jian-Ping
Liu, Min
Yao, Xu-Dong
Ma, Qing-Wei
Zheng, Jun-Hua
Yan, Yang
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-07-23
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Calcium oxalate nephrolithiasis is the most common urological disease, but noninvasive and convenient methods of diagnosis are rarely available.
Objective.
The present study aimed to identify potential urine biomarkers for noninvasive diagnosis of CaOx nephrolithiasis.
Methodology.
Urine samples from 72 patients with CaOx nephrolithiasis and 30 healthy controls were collected and proteomics analysis was performed using matrix-assisted laser desorption/ionization-time of flight-mass spectrometer (MALDI-TOF-MS).
Results.
Thirteen proteins/peptides displayed statistically significant differences.
The peptides of m/z 1207.23 and 2773.86 were selected by the genetic algorithm (GA) to build a possible diagnostic model.
The area under the curve of m/z 1207.23 and 2773.86 was 0.936 and 0.987, respectively.
The diagnostic model in distinguishing patients and healthy subjects showed 100% sensitivity and specificity.
The peak at m/z 2773.86 was identified as fibrinogen alpha chain (FGA) with the sequence G.EGDFLAEGGGVR.G, and the peak at m/z 2773.86 was identified as apolipoprotein A-I (apoA-I) with the sequence L.PVLESFKVSFLSALEEYTKKLNTQ.
Conclusion.
The study results strongly suggested that urinary FGA and apoA-I are highly sensitive and specific biomarkers for noninvasive diagnosis of CaOx nephrolithiasis.
American Psychological Association (APA)
Zhu, Wei& Liu, Min& Wang, Guang-Chun& Peng, Bo& Yan, Yang& Che, Jian-Ping…[et al.]. 2014. Fibrinogen Alpha Chain Precursor and Apolipoprotein A-I in Urine as Biomarkers for Noninvasive Diagnosis of Calcium Oxalate Nephrolithiasis : A Proteomics Study. BioMed Research International،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-470366
Modern Language Association (MLA)
Zhu, Wei…[et al.]. Fibrinogen Alpha Chain Precursor and Apolipoprotein A-I in Urine as Biomarkers for Noninvasive Diagnosis of Calcium Oxalate Nephrolithiasis : A Proteomics Study. BioMed Research International No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-470366
American Medical Association (AMA)
Zhu, Wei& Liu, Min& Wang, Guang-Chun& Peng, Bo& Yan, Yang& Che, Jian-Ping…[et al.]. Fibrinogen Alpha Chain Precursor and Apolipoprotein A-I in Urine as Biomarkers for Noninvasive Diagnosis of Calcium Oxalate Nephrolithiasis : A Proteomics Study. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-470366
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-470366