Pharmacokinetics and Biodistribution Study of 7A7 Anti-Mouse Epidermal Growth Factor Receptor Monoclonal Antibody and Its F(ab’)2 Fragment in an Immunocompetent Mouse Model
Joint Authors
López, Armando
González, Jorge Ernesto
Rabasa Capote, Ailem
Sánchez Ramírez, Belinda
Garrido Hidalgo, Greta
Rodríguez-Vera, Leyanis
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-11-21
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Pharmacy, Health & Medical Sciences
Pharmacology
Abstract EN
Immunocompetent mice, Fc receptor γ-chain deficient mice (Fcer1g−/−), and molecular tools as F(ab′)2 bivalent fragments appear as the most suitable biological models to study the mechanisms of the action of anti-epidermal growth factor receptor (EGFR) monoclonal antibodies (mAbs).
In vivo experiments contrasting antitumor effects of whole Abs and their bivalent fragments commonly involve a previous comparative pharmacokinetics study.
In this paper, pharmacokinetics and biodistribution of an anti-mouse EGFR Ab were assessed using immunocompetent mice.
125I-labeled 7A7 mAb holds an elimination half-life (t1/2β) of 23.1 h in C57BL/6 mice.
Accumulation of mAb was found in liver, spleen, kidneys, and mostly in lungs.
We used an ELISA method to determine the t1/2β of a 7A7 mAb using the same experimental setting.
Results from this new analysis revealed a t1/2β of 23.9 h, supporting this method as a safer and easier system to evaluate pharmacokinetics parameters of mAbs targeting mouse EGFR.
Using this system we also studied pharmacokinetics of 7A7 F(ab′)2 fragment.
A tenfold difference between the mAb and fragment t1/2β was found.
These data support the use of the 7A7 F(ab′)2 fragment in in vivo studies to explore the contribution of the EGFR signaling blockade and the Fc region to the antitumor effect of 7A7 mAb in this autologous scenario.
American Psychological Association (APA)
Rabasa Capote, Ailem& González, Jorge Ernesto& Rodríguez-Vera, Leyanis& López, Armando& Sánchez Ramírez, Belinda& Garrido Hidalgo, Greta. 2012. Pharmacokinetics and Biodistribution Study of 7A7 Anti-Mouse Epidermal Growth Factor Receptor Monoclonal Antibody and Its F(ab’)2 Fragment in an Immunocompetent Mouse Model. ISRN Pharmacology،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-470548
Modern Language Association (MLA)
Rabasa Capote, Ailem…[et al.]. Pharmacokinetics and Biodistribution Study of 7A7 Anti-Mouse Epidermal Growth Factor Receptor Monoclonal Antibody and Its F(ab’)2 Fragment in an Immunocompetent Mouse Model. ISRN Pharmacology No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-470548
American Medical Association (AMA)
Rabasa Capote, Ailem& González, Jorge Ernesto& Rodríguez-Vera, Leyanis& López, Armando& Sánchez Ramírez, Belinda& Garrido Hidalgo, Greta. Pharmacokinetics and Biodistribution Study of 7A7 Anti-Mouse Epidermal Growth Factor Receptor Monoclonal Antibody and Its F(ab’)2 Fragment in an Immunocompetent Mouse Model. ISRN Pharmacology. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-470548
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-470548