Characterization of Major Surface Protease Homologues of Trypanosoma congolense
Joint Authors
Marcoux, Veronica
Bull, Harold J.
Wei, Guojian
Tabel, Henry
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-01-20
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
Trypanosomes encode a family of proteins known as Major Surface Metalloproteases (MSPs).
We have identified six putative MSPs encoded within the partially sequenced T.
congolense genome.
Phylogenic analysis indicates that T.
congolense MSPs belong to five subfamilies that are conserved among African trypanosome species.
Molecular modeling, based on the known structure of Leishmania Major GP63, reveals subfamily-specific structural variations around the putative active site despite conservation of overall structure, suggesting that each MSP subfamily has evolved to recognize distinct substrates.
We have cloned and purified a protein encoding the amino-terminal domain of the T.
congolense homologue TcoMSP-D (most closely related to Leishmania GP63).
We detect TcoMSP-D in the serum of T.
congolense-infected mice.
Mice immunized with the amino-terminal domain of TcoMSP-D generate a persisting IgG1 antibody response.
Surprisingly, a low-dose challenge of immunized mice with T.
congolense significantly increases susceptibility to infection, indicating that immunity to TcoMSP-D is a factor affecting virulence.
American Psychological Association (APA)
Marcoux, Veronica& Wei, Guojian& Tabel, Henry& Bull, Harold J.. 2010. Characterization of Major Surface Protease Homologues of Trypanosoma congolense. Journal of Biomedicine and Biotechnology،Vol. 2010, no. 2010, pp.1-14.
https://search.emarefa.net/detail/BIM-470618
Modern Language Association (MLA)
Marcoux, Veronica…[et al.]. Characterization of Major Surface Protease Homologues of Trypanosoma congolense. Journal of Biomedicine and Biotechnology No. 2010 (2010), pp.1-14.
https://search.emarefa.net/detail/BIM-470618
American Medical Association (AMA)
Marcoux, Veronica& Wei, Guojian& Tabel, Henry& Bull, Harold J.. Characterization of Major Surface Protease Homologues of Trypanosoma congolense. Journal of Biomedicine and Biotechnology. 2010. Vol. 2010, no. 2010, pp.1-14.
https://search.emarefa.net/detail/BIM-470618
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-470618