Determination of Polyphenol Components of Korean Prostrate Spurge (Euphorbia supina) by Using Liquid Chromatography—Tandem Mass Spectrometry : Overall Contribution to Antioxidant Activity
Joint Authors
Kim, Chi-Yeon
Park, Semin
Kim, Yun-Hi
Lee, Won-Sup
Song, Yi
Jin, Jong Sung
Shin, Sung-Chul
Lee, Soo Jung
Kim, Gon-Sup
Bark, Ki-Min
Lee, Ji Eun
Jeong, Sung Woo
Ok, Se Yun
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-03-03
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
The Korean prostrate spurge Euphorbia supina is a weed that has been used in folk medicine in Korea against a variety of diseases.
Nine polyphenols were characterized for this plant by using high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) and the results were compared with the literature data.
The individual components were validated using the calibration curves of structurally related external standards and quantified for the first time by using the validated method.
Correlation coefficients (r2) were >0.9907.
The limit of detection and limit of quantification of the method were >0.028 mg/L and 0.094 mg/L, respectively.
Recoveries measured at 50 mg/L and 100 mg/L were 76.1–102.8% and 85.2–98.6%, respectively.
The total amount of the identified polyphenols was 3352.9 ± 2.8 mg/kg fresh plant.
Quercetin and kaempferol derivatives formed 84.8% of the total polyphenols.
The antioxidant activities of the flavonoids were evaluated in terms of 1,1-diphenyl-2-picrylhydrazyl and 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) radical cation-scavenging activity, and the reducing power showed a dose-dependent increase.
Cell viability was effectively suppressed at polyphenol mixture concentrations >250 mg/L.
American Psychological Association (APA)
Song, Yi& Jeong, Sung Woo& Lee, Won-Sup& Park, Semin& Kim, Yun-Hi& Kim, Gon-Sup…[et al.]. 2014. Determination of Polyphenol Components of Korean Prostrate Spurge (Euphorbia supina) by Using Liquid Chromatography—Tandem Mass Spectrometry : Overall Contribution to Antioxidant Activity. Journal of Analytical Methods in Chemistry،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-470667
Modern Language Association (MLA)
Song, Yi…[et al.]. Determination of Polyphenol Components of Korean Prostrate Spurge (Euphorbia supina) by Using Liquid Chromatography—Tandem Mass Spectrometry : Overall Contribution to Antioxidant Activity. Journal of Analytical Methods in Chemistry No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-470667
American Medical Association (AMA)
Song, Yi& Jeong, Sung Woo& Lee, Won-Sup& Park, Semin& Kim, Yun-Hi& Kim, Gon-Sup…[et al.]. Determination of Polyphenol Components of Korean Prostrate Spurge (Euphorbia supina) by Using Liquid Chromatography—Tandem Mass Spectrometry : Overall Contribution to Antioxidant Activity. Journal of Analytical Methods in Chemistry. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-470667
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-470667