Viability Reagent, PrestoBlue, in Comparison with Other Available Reagents, Utilized in Cytotoxicity and Antimicrobial Assays
Joint Authors
Henley-Smith, Cynthia Joan
Berrington, Danielle
De Canha, Marco Nuno
Lall, Namrita
Oosthuizen, Carel Basson
Source
International Journal of Microbiology
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-04-04
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Medicine
Abstract EN
This study compared different commercially available viability reagents.
The growth indicator reagents include p-iodonitrotetrazolium violet (INT), PrestoBlue, and Alamar Blue which were used for antimicrobial analysis against Streptococcus mutans, Prevotella intermedia, Propionibacterium acnes, and Mycobacterium tuberculosis.
PrestoBlue and Alamar Blue are resazurin based reagents that resulted in a quick and easily distinguishable colour change that allowed for visual readings.
INT and Sodium 3′-[1-(phenyl amino-carbonyl)-3,4-tetrazolium]-bis-[4-methoxy-6-nitro] benzene sulfonic acid hydrate (XTT) are tetrazolium based reagents which are converted to a formazan dye in the presence of metabolically active mitochondria enzyme.
For cell viability analysis, reagents XTT and PrestoBlue were compared.
PrestoBlue was able to clearly indicate the minimum inhibitory concentration (MIC) of various positive drug controls on various microbial strains.
PrestoBlue was also a good indicator of the 50% inhibitory concentration (IC50) of positive drug controls on various cell lines.
American Psychological Association (APA)
Lall, Namrita& Henley-Smith, Cynthia Joan& De Canha, Marco Nuno& Oosthuizen, Carel Basson& Berrington, Danielle. 2013. Viability Reagent, PrestoBlue, in Comparison with Other Available Reagents, Utilized in Cytotoxicity and Antimicrobial Assays. International Journal of Microbiology،Vol. 2013, no. 2013, pp.1-5.
https://search.emarefa.net/detail/BIM-470834
Modern Language Association (MLA)
Lall, Namrita…[et al.]. Viability Reagent, PrestoBlue, in Comparison with Other Available Reagents, Utilized in Cytotoxicity and Antimicrobial Assays. International Journal of Microbiology No. 2013 (2013), pp.1-5.
https://search.emarefa.net/detail/BIM-470834
American Medical Association (AMA)
Lall, Namrita& Henley-Smith, Cynthia Joan& De Canha, Marco Nuno& Oosthuizen, Carel Basson& Berrington, Danielle. Viability Reagent, PrestoBlue, in Comparison with Other Available Reagents, Utilized in Cytotoxicity and Antimicrobial Assays. International Journal of Microbiology. 2013. Vol. 2013, no. 2013, pp.1-5.
https://search.emarefa.net/detail/BIM-470834
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-470834