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Calibration of Surface Plasmon Resonance Imager for Biochemical Detection
Joint Authors
Abdelghani, A.
Abdelghani, Adnane
Baccar, H.
Ktari, T.
Source
International Journal of Electrochemistry
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-11-16
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
We present a new Surface Plasmon Resonance imager (SPRi) based on immobilized T4-phage for bacteria detection.
First, we present the sensitivity of the SPR imager towards refractive index variation for biosensor application.
The SPR imager can be calibrated versus different percentage of triethylene glycol mixture in ultrapure water.
The system can be used as a refractometer with sensitivity below 5×10−5 in the range of 1.33300–1.34360.
Second, bacteriophage (T4-phage) can be physisorbed on gold microarray spots for bacteria detection.
The kinetic physisorption of different concentrations of T4-phages can be observed in real time.
Finally, two types of bacteria such as E.
coli (gram negative) and Lactobacillus (gram positive) were used for positive and negative tests.
The results show a selectivity of T4-phage toward E.
coli with a detection limit below 104 CFU/mL and with good reproducibility.
American Psychological Association (APA)
Ktari, T.& Baccar, H.& Abdelghani, Adnane& Abdelghani, A.. 2011. Calibration of Surface Plasmon Resonance Imager for Biochemical Detection. International Journal of Electrochemistry،Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-470952
Modern Language Association (MLA)
Ktari, T.…[et al.]. Calibration of Surface Plasmon Resonance Imager for Biochemical Detection. International Journal of Electrochemistry No. 2012 (2012), pp.1-5.
https://search.emarefa.net/detail/BIM-470952
American Medical Association (AMA)
Ktari, T.& Baccar, H.& Abdelghani, Adnane& Abdelghani, A.. Calibration of Surface Plasmon Resonance Imager for Biochemical Detection. International Journal of Electrochemistry. 2011. Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-470952
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-470952