A High-Throughput Solid-Phase Microplate Protein-Binding Assay to Investigate Interactions between Myofilament Proteins
Joint Authors
Biesiadecki, Brandon J.
Jin, J.-P.
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-11-13
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
To understand the structure-function relationship of muscle-regulatory-protein isoforms, mutations, and posttranslational modifications, it is necessary to probe functional effects at the level of the protein-protein interaction.
Traditional methodologies assessing such protein-protein interactions are laborious and require significant amounts of purified protein, while many current methodologies require costly and specialized equipment or modification of the proteins, which may affect their interaction.
To address these issues, we developed a novel method of microplate-based solid-phase protein-binding assay over the recent years.
This method assesses specific protein-protein interactions at physiological conditions, utilizes relatively small amounts of protein, is free of protein modification, and does not require specialized instrumentation.
Here we present detailed methodology for the solid-phase protein-binding assay with examples that we have successfully applied to quantify interactions of myofilament-regulatory proteins.
We further provide considerations for optimization of the assay conditions and its broader application in studies of other protein-protein interactions.
American Psychological Association (APA)
Biesiadecki, Brandon J.& Jin, J.-P.. 2011. A High-Throughput Solid-Phase Microplate Protein-Binding Assay to Investigate Interactions between Myofilament Proteins. Journal of Biomedicine and Biotechnology،Vol. 2011, no. 2011, pp.1-8.
https://search.emarefa.net/detail/BIM-470953
Modern Language Association (MLA)
Biesiadecki, Brandon J.& Jin, J.-P.. A High-Throughput Solid-Phase Microplate Protein-Binding Assay to Investigate Interactions between Myofilament Proteins. Journal of Biomedicine and Biotechnology No. 2011 (2011), pp.1-8.
https://search.emarefa.net/detail/BIM-470953
American Medical Association (AMA)
Biesiadecki, Brandon J.& Jin, J.-P.. A High-Throughput Solid-Phase Microplate Protein-Binding Assay to Investigate Interactions between Myofilament Proteins. Journal of Biomedicine and Biotechnology. 2011. Vol. 2011, no. 2011, pp.1-8.
https://search.emarefa.net/detail/BIM-470953
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-470953