Preparation of DNA Ladder Based on Multiplex PCR Technique
Joint Authors
Guo, Li li
Zhang, Jun-he
Wang, Tian-Yun
Source
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-3, 3 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-07-25
Country of Publication
Egypt
No. of Pages
3
Main Subjects
Abstract EN
DNA molecular weight standard control, also called DNA marker (ladder), has been widely used in the experiments of molecular biology.
In the paper, we report a method by which DNA marker was prepared based on multiple PCR technique.
100–1000 bp DNA fragments were amplified using the primers designed according to the 6631 ~ 7630 position of lambda DNA.
Target DNA fragments were amplified using Touchdown PCR combined with hot start PCR, respectively, followed extracted by phenol/chloroform, precipitated with ethanol and mixed thoroughly.
The results showed that the 100–1000 bp DNA fragments were successfully obtained in one PCR reaction, the bands of prepared DNA marker were clear, the size was right and could be used as control in the molecular biology experiment.
This method could save time and be more inexpensive, rapid, simple when compared with the current DNA Ladder prepared means.
American Psychological Association (APA)
Wang, Tian-Yun& Guo, Li li& Zhang, Jun-he. 2010. Preparation of DNA Ladder Based on Multiplex PCR Technique. Journal of Nucleic Acids،Vol. 2010, no. 2010, pp.1-3.
https://search.emarefa.net/detail/BIM-470962
Modern Language Association (MLA)
Wang, Tian-Yun…[et al.]. Preparation of DNA Ladder Based on Multiplex PCR Technique. Journal of Nucleic Acids No. 2010 (2010), pp.1-3.
https://search.emarefa.net/detail/BIM-470962
American Medical Association (AMA)
Wang, Tian-Yun& Guo, Li li& Zhang, Jun-he. Preparation of DNA Ladder Based on Multiplex PCR Technique. Journal of Nucleic Acids. 2010. Vol. 2010, no. 2010, pp.1-3.
https://search.emarefa.net/detail/BIM-470962
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-470962