Micellar Liquid Chromatography Determination of Spermine in Fish Sauce after Derivatization with 3,5-Dinitrobenzoyl Chloride

Joint Authors

Esteve-Romero, Josep
Peris-Vicente, Juan
Chin-Chen, Mei-Liang
Carda-Broch, Samuel
Rambla-Alegre, Maria

Source

Chromatography Research International

Issue

Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-6, 6 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2012-03-27

Country of Publication

Egypt

No. of Pages

6

Main Subjects

Chemistry

Abstract EN

A practical liquid chromatographic method has been developed for the selective determination of the levels of spermine in anchovy sauce after derivatization with 3,5 dinitrobenzoyl chloride.

The micellar liquid chromatographic separation proposed here uses a C18 column (125×4.6 mm), followed by detection of spermine derivative at 260 nm.

Elution of the analyte was performed using a mobile phase of 0.15 M SDS-4% (v/v) 1-pentanol-pH 7 running under isocratic mode at 25°C.

Validation parameters were linearity (2–100 μg/mL, R2>0.999), detection and quantification limits (0.4 and 1.2 μg/mL, resp.), precision (less than 3.6%), accuracy (93.3–101.1%), and robustness (less than 4.8%).

These results are in agreement with the requirements of the FDA guidelines.

The proposed method was successfully applied to the monitorization of spermine formation in unsalted and salted fish sauce samples.

The suggested methodology was found useful in routine analysis of spermine in fish sauce samples.

American Psychological Association (APA)

Chin-Chen, Mei-Liang& Rambla-Alegre, Maria& Carda-Broch, Samuel& Esteve-Romero, Josep& Peris-Vicente, Juan. 2012. Micellar Liquid Chromatography Determination of Spermine in Fish Sauce after Derivatization with 3,5-Dinitrobenzoyl Chloride. Chromatography Research International،Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-470970

Modern Language Association (MLA)

Chin-Chen, Mei-Liang…[et al.]. Micellar Liquid Chromatography Determination of Spermine in Fish Sauce after Derivatization with 3,5-Dinitrobenzoyl Chloride. Chromatography Research International No. 2012 (2012), pp.1-6.
https://search.emarefa.net/detail/BIM-470970

American Medical Association (AMA)

Chin-Chen, Mei-Liang& Rambla-Alegre, Maria& Carda-Broch, Samuel& Esteve-Romero, Josep& Peris-Vicente, Juan. Micellar Liquid Chromatography Determination of Spermine in Fish Sauce after Derivatization with 3,5-Dinitrobenzoyl Chloride. Chromatography Research International. 2012. Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-470970

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-470970