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Phylogenetic, Molecular, and Biochemical Characterization of Caffeic Acid o-Methyltransferase Gene Family in Brachypodium distachyon
Joint Authors
Wu, Jiajie
Anderson, Olin
Bragg, Jennifer
Gu, Yong Q.
Vogel, John
Luo, Yangfan
Wu, Xianting
Source
International Journal of Plant Genomics
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-01-17
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Caffeic acid o-methyltransferase (COMT) is one of the important enzymes controlling lignin monomer production in plant cell wall synthesis.
Analysis of the genome sequence of the new grass model Brachypodium distachyon identified four COMT gene homologs, designated as BdCOMT1, BdCOMT2, BdCOMT3, and BdCOMT4.
Phylogenetic analysis suggested that they belong to the COMT gene family, whereas syntenic analysis through comparisons with rice and sorghum revealed that BdCOMT4 on Chromosome 3 is the orthologous copy of the COMT genes well characterized in other grass species.
The other three COMT genes are unique to Brachypodium since orthologous copies are not found in the collinear regions of rice and sorghum genomes.
Expression studies indicated that all four Brachypodium COMT genes are transcribed but with distinct patterns of tissue specificity.
Full-length cDNAs were cloned in frame into the pQE-T7 expression vector for the purification of recombinant Brachypodium COMT proteins.
Biochemical characterization of enzyme activity and substrate specificity showed that BdCOMT4 has significant effect on a broad range of substrates with the highest preference for caffeic acid.
The other three COMTs had low or no effect on these substrates, suggesting that a diversified evolution occurred on these duplicate genes that not only impacted their pattern of expression, but also altered their biochemical properties.
American Psychological Association (APA)
Wu, Xianting& Wu, Jiajie& Luo, Yangfan& Bragg, Jennifer& Anderson, Olin& Vogel, John…[et al.]. 2013. Phylogenetic, Molecular, and Biochemical Characterization of Caffeic Acid o-Methyltransferase Gene Family in Brachypodium distachyon. International Journal of Plant Genomics،Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-470991
Modern Language Association (MLA)
Wu, Xianting…[et al.]. Phylogenetic, Molecular, and Biochemical Characterization of Caffeic Acid o-Methyltransferase Gene Family in Brachypodium distachyon. International Journal of Plant Genomics No. 2013 (2013), pp.1-12.
https://search.emarefa.net/detail/BIM-470991
American Medical Association (AMA)
Wu, Xianting& Wu, Jiajie& Luo, Yangfan& Bragg, Jennifer& Anderson, Olin& Vogel, John…[et al.]. Phylogenetic, Molecular, and Biochemical Characterization of Caffeic Acid o-Methyltransferase Gene Family in Brachypodium distachyon. International Journal of Plant Genomics. 2013. Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-470991
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-470991