Inhibitory Effect of Natural Anti-Inflammatory Compounds on Cytokines Released by Chronic Venous Disease Patient-Derived Endothelial Cells
Joint Authors
Rimondi, Erika
Secchiero, Paola
Brunelli, Laura
Menegatti, Erica
Tisato, Veronica
Gianesini, Sergio
Zamboni, Paolo
Zauli, Giorgio
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-12-31
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Large vein endothelium plays important roles in clinical diseases such as chronic venous disease (CVD) and thrombosis; thus to characterize CVD vein endothelial cells (VEC) has a strategic role in identifying specific therapeutic targets.
On these bases we evaluated the effect of the natural anti-inflammatory compounds α-Lipoic acid and Ginkgoselect phytosome on cytokines/chemokines released by CVD patient-derived VEC.
For this purpose, we characterized the levels of a panel of cytokines/chemokines (n=31) in CVD patients’ plasma compared to healthy controls and their release by VEC purified from the same patients, in unstimulated and TNF-α stimulated conditions.
Among the cytokines/chemokines released by VEC, which recapitulated the systemic profile (IL-8, TNF-α, GM-CSF, INF-α2, G-CSF, MIP-1β, VEGF, EGF, Eotaxin, MCP-1, CXCL10, PDGF, and RANTES), we identified those targeted by ex vivo treatment with α-Lipoic acid and/or Ginkgoselect phytosome (GM-CSF, G-CSF, CXCL10, PDGF, and RANTES).
Finally, by investigating the intracellular pathways involved in promoting the VEC release of cytokines/chemokines, which are targeted by natural anti-inflammatory compounds, we documented that α-Lipoic acid significantly counteracted TNF-α-induced NF-κB and p38/MAPK activation while the effects of Ginkgo biloba appeared to be predominantly mediated by Akt.
Our data provide new insights into the molecular mechanisms of CVD pathogenesis, highlighting new potential therapeutic targets.
American Psychological Association (APA)
Tisato, Veronica& Zauli, Giorgio& Rimondi, Erika& Gianesini, Sergio& Brunelli, Laura& Menegatti, Erica…[et al.]. 2013. Inhibitory Effect of Natural Anti-Inflammatory Compounds on Cytokines Released by Chronic Venous Disease Patient-Derived Endothelial Cells. Mediators of Inflammation،Vol. 2013, no. 2013, pp.1-13.
https://search.emarefa.net/detail/BIM-471002
Modern Language Association (MLA)
Tisato, Veronica…[et al.]. Inhibitory Effect of Natural Anti-Inflammatory Compounds on Cytokines Released by Chronic Venous Disease Patient-Derived Endothelial Cells. Mediators of Inflammation No. 2013 (2013), pp.1-13.
https://search.emarefa.net/detail/BIM-471002
American Medical Association (AMA)
Tisato, Veronica& Zauli, Giorgio& Rimondi, Erika& Gianesini, Sergio& Brunelli, Laura& Menegatti, Erica…[et al.]. Inhibitory Effect of Natural Anti-Inflammatory Compounds on Cytokines Released by Chronic Venous Disease Patient-Derived Endothelial Cells. Mediators of Inflammation. 2013. Vol. 2013, no. 2013, pp.1-13.
https://search.emarefa.net/detail/BIM-471002
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-471002