Development and Validation of an RP-HPLC Method for Estimation of Chlorpheniramine Maleate, Ibuprofen, and Phenylephrine Hydrochloride in Combined Pharmaceutical Dosage Form
Joint Authors
Uchadadiya, Nirav B.
Mehta, Falgun A.
Sanchaniya, Pinak M.
Source
Chromatography Research International
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-07-24
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
The objective of this paper is to develope a simple, precise, accurate, and reproducible reversed phase high performance liquid chromatographic method for the quantitative determination of chlorpheniramine maleate, ibuprofen, and phenylephrine hydrochloride in combined pharmaceutical dosage form.
Analysis was carried out using acetonitrile : mathanol : phoshphate buffer (50 : 20 : 30, v/v/v, pH 5.6) mobile phase at 1.0 mL/min flow rate and Sunfire C 18 column (5 μm × 250 mm × 4.6 mm) as stationary phase with detection wavelength of 220 nm.
The retention times of chlorpheniramine maleate (CPM), ibuprofen (IBU), and phenylephrine hydrochloride (PHE) were 4.2 min, 13.6 min, and 2.7 min, respectively.
The proposed method was validated with respect to linearity, accuracy, precision, specificity, and robustness.
The linearity for chlorpheniramine maleate, ibuprofen, and phenylephrine hydrochloride was in the range of 0.5–2.5 μg/mL, 25–125 μg/mL, and 1.25–6.25 μg/mL, respectively.
The % recoveries of all the three drugs were found to be 99.44–101.61%, 99.39–101.79%, and 98.66–101.83%.
LOD were found to be 32, 120, and 68 ng/mL for CPM, IBU, and PHE, respectively.
The method was successfully applied to the estimation of chlorpheniramine maleate, ibuprofen, and phenylephrine hydrochloride in combined pharmaceutical dosage form.
American Psychological Association (APA)
Sanchaniya, Pinak M.& Mehta, Falgun A.& Uchadadiya, Nirav B.. 2013. Development and Validation of an RP-HPLC Method for Estimation of Chlorpheniramine Maleate, Ibuprofen, and Phenylephrine Hydrochloride in Combined Pharmaceutical Dosage Form. Chromatography Research International،Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-471136
Modern Language Association (MLA)
Sanchaniya, Pinak M.…[et al.]. Development and Validation of an RP-HPLC Method for Estimation of Chlorpheniramine Maleate, Ibuprofen, and Phenylephrine Hydrochloride in Combined Pharmaceutical Dosage Form. Chromatography Research International No. 2013 (2013), pp.1-6.
https://search.emarefa.net/detail/BIM-471136
American Medical Association (AMA)
Sanchaniya, Pinak M.& Mehta, Falgun A.& Uchadadiya, Nirav B.. Development and Validation of an RP-HPLC Method for Estimation of Chlorpheniramine Maleate, Ibuprofen, and Phenylephrine Hydrochloride in Combined Pharmaceutical Dosage Form. Chromatography Research International. 2013. Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-471136
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-471136