Comparison of a New Gold Immunochromatographic Assay for the Rapid Diagnosis of the Novel Influenza A (H7N9)‎ Virus with Cell Culture and a Real-Time Reverse-Transcription PCR Assay

Joint Authors

Yao, Hangping
Chen, Yu
Kang, Keren
Wu, Haibo
Jin, Changzhong
Tang, Shixing
Peng, Xiaorong
Wu, Nanping
Liu, Fumin
Lu, Xiangyun
Li, Lanjuan
Cheng, Linfang
Xie, Tiansheng

Source

BioMed Research International

Issue

Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-6, 6 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2014-04-14

Country of Publication

Egypt

No. of Pages

6

Main Subjects

Medicine

Abstract EN

We assessed a colloidal gold immunochromatographic assay (GICA) for rapid detection of influenza A (H7N9) and compared it with reverse-transcription-polymerase chain reaction (RT-PCR) and viral culture.

Samples from 35 H7N9 infected patients were collected, including 45 throat swab samples, 56 sputum samples, and 39 feces samples.

All samples were tested by GICA, viral culture, and RT-PCR.

GICA specifically reacted with recombinant HA proteins, virus lysates, and clinical samples from H7 subtype viruses.

Compared with RT-PCR, GICA demonstrated low sensitivity (33.33%) but high specificity (97.56%).

The positive rate of GICA tests for samples collected in the period from 8 to 21 days after contact with poultry was much higher than those for samples collected before or after this period.

Compared with viral culture, GICA showed sensitivity of 91.67% and specificity of 82.03%.

Sputum specimens were more likely to test positive for H7N9 virus than samples from throat swabs and feces.

The GICA-based H7 test is a reliable, rapid, and convenient method for the screening and diagnosis of influenza A (H7N9) disease, especially for the sputum specimens with high viral load.

It may be helpful in managing H7N9 epidemics and preliminary diagnosis in early stages in resource-limited settings.

American Psychological Association (APA)

Jin, Changzhong& Wu, Nanping& Peng, Xiaorong& Yao, Hangping& Lu, Xiangyun& Chen, Yu…[et al.]. 2014. Comparison of a New Gold Immunochromatographic Assay for the Rapid Diagnosis of the Novel Influenza A (H7N9) Virus with Cell Culture and a Real-Time Reverse-Transcription PCR Assay. BioMed Research International،Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-471149

Modern Language Association (MLA)

Jin, Changzhong…[et al.]. Comparison of a New Gold Immunochromatographic Assay for the Rapid Diagnosis of the Novel Influenza A (H7N9) Virus with Cell Culture and a Real-Time Reverse-Transcription PCR Assay. BioMed Research International No. 2014 (2014), pp.1-6.
https://search.emarefa.net/detail/BIM-471149

American Medical Association (AMA)

Jin, Changzhong& Wu, Nanping& Peng, Xiaorong& Yao, Hangping& Lu, Xiangyun& Chen, Yu…[et al.]. Comparison of a New Gold Immunochromatographic Assay for the Rapid Diagnosis of the Novel Influenza A (H7N9) Virus with Cell Culture and a Real-Time Reverse-Transcription PCR Assay. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-471149

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-471149