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Towards a Systems Approach in the Genetic Analysis of Archaea : Accelerating Mutant Construction and Phenotypic Analysis in Haloferax volcanii
Joint Authors
Phillips, Gabriela
Blaby-Haas, Crysten E.
de Crécy-Lagard, Valérie
El Yacoubi, Basma
Gulig, Kevin S.
Blaby, Ian K.
Source
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-12-23
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
With the availability of a genome sequence and increasingly sophisticated genetic tools, Haloferax volcanii is becoming a model for both Archaea and halophiles.
In order for H.
volcanii to reach a status equivalent to Escherichia coli, Bacillus subtilis, or Saccharomyces cerevisiae, a gene knockout collection needs to be constructed in order to identify the archaeal essential gene set and enable systematic phenotype screens.
A streamlined gene-deletion protocol adapted for potential automation was implemented and used to generate 22 H.
volcanii deletion strains and identify several potentially essential genes.
These gene deletion mutants, generated in this and previous studies, were then analyzed in a high-throughput fashion to measure growth rates in different media and temperature conditions.
We conclude that these high-throughput methods are suitable for a rapid investigation of an H.
volcanii mutant library and suggest that they should form the basis of a larger genome-wide experiment.Erratum of Towards a Systems Approach in the Genetic Analysis of Archaea: Accelerating Mutant Construction and Phenotypic Analysis in Haloferax volcanii dx.doi.org/10.1155/2011/867406
American Psychological Association (APA)
Blaby, Ian K.& Phillips, Gabriela& Blaby-Haas, Crysten E.& Gulig, Kevin S.& El Yacoubi, Basma& de Crécy-Lagard, Valérie. 2010. Towards a Systems Approach in the Genetic Analysis of Archaea : Accelerating Mutant Construction and Phenotypic Analysis in Haloferax volcanii. Archaea،Vol. 2010, no. 2010, pp.1-11.
https://search.emarefa.net/detail/BIM-471266
Modern Language Association (MLA)
Blaby, Ian K.…[et al.]. Towards a Systems Approach in the Genetic Analysis of Archaea : Accelerating Mutant Construction and Phenotypic Analysis in Haloferax volcanii. Archaea No. 2010 (2010), pp.1-11.
https://search.emarefa.net/detail/BIM-471266
American Medical Association (AMA)
Blaby, Ian K.& Phillips, Gabriela& Blaby-Haas, Crysten E.& Gulig, Kevin S.& El Yacoubi, Basma& de Crécy-Lagard, Valérie. Towards a Systems Approach in the Genetic Analysis of Archaea : Accelerating Mutant Construction and Phenotypic Analysis in Haloferax volcanii. Archaea. 2010. Vol. 2010, no. 2010, pp.1-11.
https://search.emarefa.net/detail/BIM-471266
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-471266