In Vivo Tracking of Murine Adipose Tissue-Derived Multipotent Adult Stem Cells and Ex Vivo Cross-Validation
Joint Authors
Lorenzon, Andrea
Cesselli, Daniela
Beltrami, Carlo Alberto
Bergamin, Natascha
Biffi, Stefania
Ferrari, Roberto
Bates, Dave
Beltrami, Antonio Paolo
Lorusso, Vito
Garrovo, Chiara
Source
International Journal of Molecular Imaging
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-01-17
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Stem cells are characterized by the ability to renew themselves and to differentiate into specialized cell types, while stem cell therapy is believed to treat a number of different human diseases through either cell regeneration or paracrine effects.
Herein, an in vivo and ex vivo near infrared time domain (NIR TD) optical imaging study was undertaken to evaluate the migratory ability of murine adipose tissue-derived multipotent adult stem cells [mAT-MASC] after intramuscular injection in mice.
In vivo NIR TD optical imaging data analysis showed a migration of DiD-labelled mAT-MASC in the leg opposite the injection site, which was confirmed by a fibered confocal microendoscopy system.
Ex vivo NIR TD optical imaging results showed a systemic distribution of labelled cells.
Considering a potential microenvironmental contamination, a cross-validation study by multimodality approaches was followed: mAT-MASC were isolated from male mice expressing constitutively eGFP, which was detectable using techniques of immunofluorescence and qPCR.
Y-chromosome positive cells, injected into wild-type female recipients, were detected by FISH.
Cross-validation confirmed the data obtained by in vivo/ex vivo TD optical imaging analysis.
In summary, our data demonstrates the usefulness of NIR TD optical imaging in tracking delivered cells, giving insights into the migratory properties of the injected cells.
American Psychological Association (APA)
Garrovo, Chiara& Bergamin, Natascha& Bates, Dave& Cesselli, Daniela& Beltrami, Antonio Paolo& Lorenzon, Andrea…[et al.]. 2013. In Vivo Tracking of Murine Adipose Tissue-Derived Multipotent Adult Stem Cells and Ex Vivo Cross-Validation. International Journal of Molecular Imaging،Vol. 2013, no. 2013, pp.1-13.
https://search.emarefa.net/detail/BIM-471330
Modern Language Association (MLA)
Garrovo, Chiara…[et al.]. In Vivo Tracking of Murine Adipose Tissue-Derived Multipotent Adult Stem Cells and Ex Vivo Cross-Validation. International Journal of Molecular Imaging No. 2013 (2013), pp.1-13.
https://search.emarefa.net/detail/BIM-471330
American Medical Association (AMA)
Garrovo, Chiara& Bergamin, Natascha& Bates, Dave& Cesselli, Daniela& Beltrami, Antonio Paolo& Lorenzon, Andrea…[et al.]. In Vivo Tracking of Murine Adipose Tissue-Derived Multipotent Adult Stem Cells and Ex Vivo Cross-Validation. International Journal of Molecular Imaging. 2013. Vol. 2013, no. 2013, pp.1-13.
https://search.emarefa.net/detail/BIM-471330
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-471330