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Differential Host Cell Gene Expression and Regulation of Cell Cycle Progression by Nonstructural Protein 11 of Porcine Reproductive and Respiratory Syndrome Virus
Joint Authors
Yoo, Dongwan
Sun, Yan
Giri, Sumanprava
Prasanth, Supriya G.
Li, Dong
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-02-26
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Nonstructural protein 11 (nsp11) of porcine reproductive and respiratory syndrome virus (PRRSV) is a viral endoribonuclease with an unknown function.
The regulation of cellular gene expression by nsp11 was examined by RNA microarrays using MARC-nsp11 cells constitutively expressing nsp11.
In these cells, the interferon-β, interferon regulatory factor 3, and nuclear factor-κB activities were suppressed compared to those of parental cells, suggesting that nsp11 might serve as a viral interferon antagonist.
Differential cellular transcriptome was examined using Affymetrix exon chips representing 28,536 transcripts, and after statistical analyses 66 cellular genes were shown to be upregulated and 104 genes were downregulated by nsp11.
These genes were grouped into 5 major signaling pathways according to their functional relations: histone-related, cell cycle and DNA replication, mitogen activated protein kinase signaling, complement, and ubiquitin-proteasome pathways.
Of these, the modulation of cell cycle by nsp11 was further investigated since many of the regulated genes fell in this particular pathway.
Flow cytometry showed that nsp11 caused the delay of cell cycle progression at the S phase and the BrdU staining confirmed the cell cycle arrest in nsp11-expressing cells.
The study provides insights into the understanding of specific cellular responses to nsp11 during PRRSV infection.
American Psychological Association (APA)
Sun, Yan& Li, Dong& Giri, Sumanprava& Prasanth, Supriya G.& Yoo, Dongwan. 2014. Differential Host Cell Gene Expression and Regulation of Cell Cycle Progression by Nonstructural Protein 11 of Porcine Reproductive and Respiratory Syndrome Virus. BioMed Research International،Vol. 2014, no. 2014, pp.1-13.
https://search.emarefa.net/detail/BIM-471676
Modern Language Association (MLA)
Sun, Yan…[et al.]. Differential Host Cell Gene Expression and Regulation of Cell Cycle Progression by Nonstructural Protein 11 of Porcine Reproductive and Respiratory Syndrome Virus. BioMed Research International No. 2014 (2014), pp.1-13.
https://search.emarefa.net/detail/BIM-471676
American Medical Association (AMA)
Sun, Yan& Li, Dong& Giri, Sumanprava& Prasanth, Supriya G.& Yoo, Dongwan. Differential Host Cell Gene Expression and Regulation of Cell Cycle Progression by Nonstructural Protein 11 of Porcine Reproductive and Respiratory Syndrome Virus. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-13.
https://search.emarefa.net/detail/BIM-471676
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-471676