Synergistic Antibacterial Effects of Polyphenolic Compounds from Olive Mill Wastewater
Joint Authors
Tafesh, Ahmed
Halahlih, Fares
Riepl, Herbert
Azaizeh, Hassan
Jadoun, Jeries
Najami, Naim
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-05-02
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Polyphenols or phenolic compounds are groups of secondary metabolites widely distributed in plants and found in olive mill wastewater (OMW).
Phenolic compounds as well as OMW extracts were evaluated in vitro for their antimicrobial activity against Gram-positive (Streptococcus pyogenes and Staphylococcus aureus) and Gram-negative bacteria (Escherichia coli and Klebsiella pneumoniae).
Most of the tested phenols were not effective against the four bacterial strains when tested as single compounds at concentrations of up to 1000 μg mL−1.
Hydroxytyrosol at 400 μg mL−1 caused complete growth inhibition of the four strains.
Gallic acid was effective at 200, and 400 μg mL−1 against S.
aureus, and S.
pyogenes, respectively, but not against the gram negative bacteria.
An OMW fraction called AntiSolvent was obtained after the addition of ethanol to the crude OMW.
HPLC analysis of AntiSolvent fraction revealed that this fraction contains mainly hydroxytyrosol (10.3%), verbascoside (7.4%), and tyrosol (2.6%).
The combinations of AntiSolvent/gallic acid were tested using the low minimal inhibitory concentrations which revealed that 50/100–100/100 μg mL−1 caused complete growth inhibition of the four strains.
These results suggest that OMW specific fractions augmented with natural phenolic ingredients may be utilized as a source of bioactive compounds to control pathogenic bacteria.
American Psychological Association (APA)
Tafesh, Ahmed& Najami, Naim& Jadoun, Jeries& Halahlih, Fares& Riepl, Herbert& Azaizeh, Hassan. 2011. Synergistic Antibacterial Effects of Polyphenolic Compounds from Olive Mill Wastewater. Evidence-Based Complementary and Alternative Medicine،Vol. 2011, no. 2011, pp.1-9.
https://search.emarefa.net/detail/BIM-471723
Modern Language Association (MLA)
Tafesh, Ahmed…[et al.]. Synergistic Antibacterial Effects of Polyphenolic Compounds from Olive Mill Wastewater. Evidence-Based Complementary and Alternative Medicine No. 2011 (2011), pp.1-9.
https://search.emarefa.net/detail/BIM-471723
American Medical Association (AMA)
Tafesh, Ahmed& Najami, Naim& Jadoun, Jeries& Halahlih, Fares& Riepl, Herbert& Azaizeh, Hassan. Synergistic Antibacterial Effects of Polyphenolic Compounds from Olive Mill Wastewater. Evidence-Based Complementary and Alternative Medicine. 2011. Vol. 2011, no. 2011, pp.1-9.
https://search.emarefa.net/detail/BIM-471723
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-471723