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Effect of Base Sequence on G-Wire Formation in Solution
Joint Authors
Ma'ani Hessari, Nason
Rigler, Martin
Drevenšek-Olenik, Irena
Spindler, Lea
Webba da Silva, Mateus
Source
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-06-03
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
The formation and dimensions of G-wires by different short G-rich DNA sequences in solution were investigated by dynamic light scattering (DLS) and polyacrilamide gel electrophoresis (PAGE).
To explore the basic principles of wire formation, we studied the effects of base sequence, method of preparation, temperature, and oligonucleotide concentration.
Both DLS and PAGE show that thermal annealing induces much less macromolecular self-assembly than dialysis.
The degree of assembly and consequently length of G-wires (5-6 nm) are well resolved by both methods for DNA sequences with intermediate length, while some discrepancies appear for the shortest and longest sequences.
As expected, the longest DNA sequence gives the longest macromolecular aggregates with a length of about 11 nm as estimated by DLS.
The quadruplex topologies show no concentration dependence in the investigated DNA concentration range (0.1 mM–0.4 mM) and no structural change upon heating.
American Psychological Association (APA)
Spindler, Lea& Rigler, Martin& Drevenšek-Olenik, Irena& Ma'ani Hessari, Nason& Webba da Silva, Mateus. 2010. Effect of Base Sequence on G-Wire Formation in Solution. Journal of Nucleic Acids،Vol. 2010, no. 2010, pp.1-8.
https://search.emarefa.net/detail/BIM-471763
Modern Language Association (MLA)
Spindler, Lea…[et al.]. Effect of Base Sequence on G-Wire Formation in Solution. Journal of Nucleic Acids No. 2010 (2010), pp.1-8.
https://search.emarefa.net/detail/BIM-471763
American Medical Association (AMA)
Spindler, Lea& Rigler, Martin& Drevenšek-Olenik, Irena& Ma'ani Hessari, Nason& Webba da Silva, Mateus. Effect of Base Sequence on G-Wire Formation in Solution. Journal of Nucleic Acids. 2010. Vol. 2010, no. 2010, pp.1-8.
https://search.emarefa.net/detail/BIM-471763
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-471763