A Stability Indicating UPLC Method for the Determination of Levofloxacin Hemihydrate in Pharmaceutical Dosage Form : Application to Pharmaceutical Analysis
Joint Authors
Jadeja, Yashwantsinh
Patel, Madhavi
Patel, Nitish
Shah, Anamik
Karia, Denish
Dabhi, Batuk
Jebaliya, Hetal
Parmar, Bhavesh
Source
Chromatography Research International
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-06-19
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
A reliable and sensitive isocratic stability indicating RP-UPLC method has been developed and validated for quantitative analysis and content uniformity study of levofloxacin hemihydrate in tablets.
An isocratic method for analysis of levofloxacin hemihydrate was archived on ACQUITY UPLC BEH C18 (100*2.1) mm particle size 1.7 μ columns within shorter runtime of 4 min with a flow rate of 0.400 mL/min and using a photodiode array detector to monitor the eluate at 294 nm.
The mobile phase consisted of acetonitrile-buffer (23 : 77 v/v), (buffer: 20 mM K2HPO4 + 1 mL triethylamine in 1 L water, pH=2.50 by orthophosphoric acid).
Response was a liner function of drug concentration in the range of 0.5–80 μg/mL (r2=0.999) with a limit of detection and quantification of 0.1 and 0.5 μg/mL, respectively.
Accuracy (recovery) was between 99.77% and 101.55%.
The drug was subjected to oxidation, hydrolysis, photolysis, and thermal degradation.
Degradation products resulting from the stress studies did not interfere with the detection of levofloxacin hemihydrate, and the assay is stability indicating.
American Psychological Association (APA)
Dabhi, Batuk& Parmar, Bhavesh& Patel, Nitish& Jadeja, Yashwantsinh& Patel, Madhavi& Jebaliya, Hetal…[et al.]. 2013. A Stability Indicating UPLC Method for the Determination of Levofloxacin Hemihydrate in Pharmaceutical Dosage Form : Application to Pharmaceutical Analysis. Chromatography Research International،Vol. 2013, no. 2013, pp.1-5.
https://search.emarefa.net/detail/BIM-471883
Modern Language Association (MLA)
Dabhi, Batuk…[et al.]. A Stability Indicating UPLC Method for the Determination of Levofloxacin Hemihydrate in Pharmaceutical Dosage Form : Application to Pharmaceutical Analysis. Chromatography Research International No. 2013 (2013), pp.1-5.
https://search.emarefa.net/detail/BIM-471883
American Medical Association (AMA)
Dabhi, Batuk& Parmar, Bhavesh& Patel, Nitish& Jadeja, Yashwantsinh& Patel, Madhavi& Jebaliya, Hetal…[et al.]. A Stability Indicating UPLC Method for the Determination of Levofloxacin Hemihydrate in Pharmaceutical Dosage Form : Application to Pharmaceutical Analysis. Chromatography Research International. 2013. Vol. 2013, no. 2013, pp.1-5.
https://search.emarefa.net/detail/BIM-471883
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-471883