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Crystal Structural and Functional Analysis of the Putative Dipeptidase from Pyrococcus horikoshii OT3
Joint Authors
Yokoyama, Shigeyuki
Ogasahara, Kyoko
Takada, Katsumi
Sawano, Masahide
Jeyakanthan, Jeyaraman
Yutani, Katsuhide
Mizutani, Hisashi
Kunishima, Naoki
Source
Issue
Vol. 2009, Issue 2009 (31 Dec. 2009), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2009-06-28
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
The crystal structure of a putative dipeptidase (Phdpd) from Pyrococcus horikoshii OT3 was solved using X-ray data at 2.4 Å resolution.
The protein is folded into two distinct entities.
The N-terminal domain consists of the general topology of the α/β fold, and the C-terminal domain consists of five long mixed strands, four helices, and two 310 helices.
The structure of Phdpd is quite similar to reported structures of prolidases from P.
furiosus (Zn-Pfprol) and P.
horikoshii (Zn-Phdpd), where Zn ions are observed in the active site resulting in an inactive form.
However, Phdpd did not contain metals in the crystal structure and showed prolidase activity in the absence of additional Co ions, whereas the specific activities increased by 5 times in the presence of a sufficient concentration (1.2 mM) of Co ions.
The substrate specificities (X-Pro) of Phdpd were broad compared with those of Zn-Phdpd in the presence of Co ions, whose relative activities are 10% or less for substrates other than Met-Pro, which is the most favorable substrate.
The binding constants of Zn-Phdpd with three metals (Zn, Co, and Mn) were higher than those of Phdpd and that with Zn was higher by greater than 2 orders, which were determined by DSC experiments.
From the structural comparison of both forms and the above experimental results, it could be elucidated why the protein with Zn2+ ions is inactive.
American Psychological Association (APA)
Jeyakanthan, Jeyaraman& Takada, Katsumi& Sawano, Masahide& Ogasahara, Kyoko& Mizutani, Hisashi& Kunishima, Naoki…[et al.]. 2009. Crystal Structural and Functional Analysis of the Putative Dipeptidase from Pyrococcus horikoshii OT3. Journal of Biophysics،Vol. 2009, no. 2009, pp.1-12.
https://search.emarefa.net/detail/BIM-471919
Modern Language Association (MLA)
Jeyakanthan, Jeyaraman…[et al.]. Crystal Structural and Functional Analysis of the Putative Dipeptidase from Pyrococcus horikoshii OT3. Journal of Biophysics No. 2009 (2009), pp.1-12.
https://search.emarefa.net/detail/BIM-471919
American Medical Association (AMA)
Jeyakanthan, Jeyaraman& Takada, Katsumi& Sawano, Masahide& Ogasahara, Kyoko& Mizutani, Hisashi& Kunishima, Naoki…[et al.]. Crystal Structural and Functional Analysis of the Putative Dipeptidase from Pyrococcus horikoshii OT3. Journal of Biophysics. 2009. Vol. 2009, no. 2009, pp.1-12.
https://search.emarefa.net/detail/BIM-471919
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-471919