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Inhibitory Effects of Arginine on the Aggregation of Bovine Insulin
Joint Authors
Newman, Jay
Varughese, Michael M.
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-07-09
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Static and dynamic light scattering were used to investigate the effects of L-arginine, commonly used to inhibit protein aggregation, on the initial aggregation kinetics of solutions of bovine insulin in 20% acetic acid and 0.1 M NaCl as a model system for amyloidosis.
Measurements were made as a function of insulin concentration (0.5–2.0 mM), quench temperature (60–85°C), and arginine concentration (10–500 mM).
Aggregation kinetics under all conditions had a lag phase, whose duration decreased with increasing temperature and with increasing insulin concentration but which increased by up to a factor of 8 with increasing added arginine.
Further, the initial growth rate after the lag phase also slowed by up to a factor of about 20 in the presence of increasing concentrations of arginine.
From the temperature dependence of the lag phase duration, we find that the nucleation activation energy doubles from 17±5 to 36±3 kcal/mol in the presence of 500 mM arginine.
American Psychological Association (APA)
Varughese, Michael M.& Newman, Jay. 2012. Inhibitory Effects of Arginine on the Aggregation of Bovine Insulin. Journal of Biophysics،Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-471935
Modern Language Association (MLA)
Varughese, Michael M.& Newman, Jay. Inhibitory Effects of Arginine on the Aggregation of Bovine Insulin. Journal of Biophysics No. 2012 (2012), pp.1-7.
https://search.emarefa.net/detail/BIM-471935
American Medical Association (AMA)
Varughese, Michael M.& Newman, Jay. Inhibitory Effects of Arginine on the Aggregation of Bovine Insulin. Journal of Biophysics. 2012. Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-471935
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-471935