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Pectinesterase Inhibitor from Jelly Fig (Ficus awkeotsang Makino) Achene Inhibits Surface Antigen Expression by Human Hepatitis B Virus
Joint Authors
Jiang, Chii-Ming
Chen, Yu-Yawn
Huang, Yu-Chuen
Chen, Yu-Jen
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-11-04
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
Pectinesterase inhibitor (PEI) isolated from jelly fig (Ficus awkeotsang Makino) is an edible component of a popular drink consumed in Asia.
Hepatitis B virus (HBV) infection is prevalent in Asia, and current treatments for HBV infection need improvement.
This study aimed to evaluate the effect of PEI on the surface antigen expression by HBV (HBsAg).
Human hepatoma cell lines Hep3B and Huh7 served as in vitro models for assessing the cytotoxicity and HBsAg expression.
A culture of primary hepatocytes cultured from mice served as the normal counterpart.
Cell viability was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) colorimetric assay.
HBsAg expression was evaluated by measuring HBsAg secretion into the culture medium using an enzyme-linked immunosorbent assay.
The results showed that PEI did not affect the viability of the human hepatoma cell lines or primary mouse hepatocytes.
PEI inhibited the expression of HBsAg in hepatoma cell lines harboring endogenous (Hep3B) and integrated (Huh7) HBV genomes in a concentration- and time-dependent manner, thus implicating a universal activity against HBV gene expression.
In conclusion, it suggests that PEI from jelly fig inhibits the expression of human HBsAg in host cells without toxic effects on normal primary hepatocytes.
American Psychological Association (APA)
Huang, Yu-Chuen& Jiang, Chii-Ming& Chen, Yu-Jen& Chen, Yu-Yawn. 2013. Pectinesterase Inhibitor from Jelly Fig (Ficus awkeotsang Makino) Achene Inhibits Surface Antigen Expression by Human Hepatitis B Virus. Evidence-Based Complementary and Alternative Medicine،Vol. 2013, no. 2013, pp.1-5.
https://search.emarefa.net/detail/BIM-471966
Modern Language Association (MLA)
Huang, Yu-Chuen…[et al.]. Pectinesterase Inhibitor from Jelly Fig (Ficus awkeotsang Makino) Achene Inhibits Surface Antigen Expression by Human Hepatitis B Virus. Evidence-Based Complementary and Alternative Medicine No. 2013 (2013), pp.1-5.
https://search.emarefa.net/detail/BIM-471966
American Medical Association (AMA)
Huang, Yu-Chuen& Jiang, Chii-Ming& Chen, Yu-Jen& Chen, Yu-Yawn. Pectinesterase Inhibitor from Jelly Fig (Ficus awkeotsang Makino) Achene Inhibits Surface Antigen Expression by Human Hepatitis B Virus. Evidence-Based Complementary and Alternative Medicine. 2013. Vol. 2013, no. 2013, pp.1-5.
https://search.emarefa.net/detail/BIM-471966
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-471966