Efficiency of Ferritin as an MRI Reporter Gene in NPC Cells Is Enhanced by Iron Supplementation
Joint Authors
Feng, Yupeng
Zhu, Junfeng
Xie, Fukang
Li, Li
Liu, Qicai
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-03-20
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Background.
An emerging MRI reporter, ferritin heavy chain (FTH1), is recently applied to enhance the contrast and increase the sensitivity of MRI in the monitoring of solid tumors.
However, FTH1-overexpression-related cytotoxicity is required to be explored.
Methods.
By using the Tet-Off system, FTH1 overexpression was semi-quantitativiely and dynamicly regulated by doxycycline in a NPC cell line.
Effects of FTH1 overexpression on the proliferation, cytotoxicity, apoptosis and migration of NPC cells were investigated in vitro, and MR relaxation rate was measured in vitro and in vivo.
Results.
In vitro and in vivo overexpression of FTH1 significantly increased the transverse relaxivity (R2), which could be enhanced by iron supplementation.
In vitro, overexpression of FTH1 reduced cell growth and migration, which were not reduced by iron supplementation.
Furthermore, cells were subcutaneously inoculated into the nude mice.
Results showed FTH1 overexpression decreased tumor growth in the absence of iron supplementation but not in the presence of iron supplementation.
Conclusion.
To maximize R2 and minimize the potential adverse effects, supplementation of iron at appropriate dose is recommended during the application of FTH1 as a reporter gene in the monitoring of NPC by MRI.
American Psychological Association (APA)
Feng, Yupeng& Liu, Qicai& Zhu, Junfeng& Xie, Fukang& Li, Li. 2012. Efficiency of Ferritin as an MRI Reporter Gene in NPC Cells Is Enhanced by Iron Supplementation. Journal of Biomedicine and Biotechnology،Vol. 2012, no. 2012, pp.1-11.
https://search.emarefa.net/detail/BIM-471970
Modern Language Association (MLA)
Feng, Yupeng…[et al.]. Efficiency of Ferritin as an MRI Reporter Gene in NPC Cells Is Enhanced by Iron Supplementation. Journal of Biomedicine and Biotechnology No. 2012 (2012), pp.1-11.
https://search.emarefa.net/detail/BIM-471970
American Medical Association (AMA)
Feng, Yupeng& Liu, Qicai& Zhu, Junfeng& Xie, Fukang& Li, Li. Efficiency of Ferritin as an MRI Reporter Gene in NPC Cells Is Enhanced by Iron Supplementation. Journal of Biomedicine and Biotechnology. 2012. Vol. 2012, no. 2012, pp.1-11.
https://search.emarefa.net/detail/BIM-471970
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-471970