The Comet Assay for the Evaluation of Genotoxic Potential of Landfill Leachate
Joint Authors
Kalka, Joanna
Widziewicz, Kamila
Madej, Paweł
Skonieczna, Magdalena
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-04-30
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Medicine
Information Technology and Computer Science
Abstract EN
Genotoxic assessment of landfill leachate before and after biological treatment was conducted with two human cell lines (Me45 and NHDF) and Daphnia magna somatic cells.
The alkali version of comet assay was used to examine genotoxicity of leachate by DNA strand breaks analysis and its repair dynamics.
The leachate samples were collected from Zabrze landfill, situated in the Upper Silesian Industrial District, Poland.
Statistically significant differences (Kruskal-Wallice ANOVA rank model) were observed between DNA strand breaks in cells incubated with leachate before and after treatment (P<0.001).
Nonparametric Friedman ANOVA confirmed time-reliable and concentration-reliable cells response to leachate concentration.
Examinations of chemical properties showed a marked decrease in leachate parameters after treatment which correlate to reduced genotoxicity towards tested cells.
Obtained results demonstrate that biological cotreatment of leachate together with municipal wastewater is an efficient method for its genotoxic potential reduction; however, treated leachate still possessed genotoxic character.
American Psychological Association (APA)
Widziewicz, Kamila& Kalka, Joanna& Skonieczna, Magdalena& Madej, Paweł. 2012. The Comet Assay for the Evaluation of Genotoxic Potential of Landfill Leachate. The Scientific World Journal،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-472010
Modern Language Association (MLA)
Widziewicz, Kamila…[et al.]. The Comet Assay for the Evaluation of Genotoxic Potential of Landfill Leachate. The Scientific World Journal No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-472010
American Medical Association (AMA)
Widziewicz, Kamila& Kalka, Joanna& Skonieczna, Magdalena& Madej, Paweł. The Comet Assay for the Evaluation of Genotoxic Potential of Landfill Leachate. The Scientific World Journal. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-472010
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-472010