Detection of Bacterial Endospores in Soil by Terbium Fluorescence
Joint Authors
Kölle, Linda
Brandl, Helmut
Brandes Ammann, Andrea
Source
International Journal of Microbiology
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-07-07
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Medicine
Abstract EN
Spore formation is a survival mechanism of microorganisms when facing unfavorable environmental conditions resulting in “dormant” states.
We investigated the occurrence of bacterial endospores in soils from various locations including grasslands (pasture, meadow), allotment gardens, and forests, as well as fluvial sediments.
Bacterial spores are characterized by their high content of dipicolinic acid (DPA).
In the presence of terbium, DPA forms a complex showing a distinctive photoluminescence spectrum.
DPA was released from soil by microwaving or autoclaving.
The addition of aluminium chloride reduced signal quenching by interfering compounds such as phosphate.
The highest spore content (up to 109 spores per gram of dry soil) was found in grassland soils.
Spore content is related to soil type, to soil depth, and to soil carbon-to-nitrogen ratio.
Our study might provide a basis for the detection of “hot spots” of bacterial spores in soil.
American Psychological Association (APA)
Brandes Ammann, Andrea& Kölle, Linda& Brandl, Helmut. 2011. Detection of Bacterial Endospores in Soil by Terbium Fluorescence. International Journal of Microbiology،Vol. 2011, no. 2011, pp.1-5.
https://search.emarefa.net/detail/BIM-472017
Modern Language Association (MLA)
Brandes Ammann, Andrea…[et al.]. Detection of Bacterial Endospores in Soil by Terbium Fluorescence. International Journal of Microbiology No. 2011 (2011), pp.1-5.
https://search.emarefa.net/detail/BIM-472017
American Medical Association (AMA)
Brandes Ammann, Andrea& Kölle, Linda& Brandl, Helmut. Detection of Bacterial Endospores in Soil by Terbium Fluorescence. International Journal of Microbiology. 2011. Vol. 2011, no. 2011, pp.1-5.
https://search.emarefa.net/detail/BIM-472017
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-472017