4-Hydroxy-17-methylincisterol from Agaricus blazei Decreased Cytokine Production and Cell Proliferation in Human Peripheral Blood Mononuclear Cells via Inhibition of NF-AT and NF-κB Activation
Joint Authors
Kuo, Yuh-Chi
Huang, Yu-Ling
Tsai, Wei-Jern
Chuang, Kai-An
Chen, Chien-Chih
Yang, Shih-Chien
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-02-27
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
Agaricus blazei Murill is an edible and medicinal mushroom.
In the previous study, we have proved that extracts of A.
blazei inhibit human peripheral blood mononuclear cell (PBMC) proliferation activated with phytohemagglutinin (PHA).
Currently, we purified 4-hydroxy-17-methylincisterol (4-HM; C21H33O3) from A.
blazei investigated its regulatory effects on cytokine productions and cell proliferation of PBMC induced by PHA.
The results indicated that 4-HM suppressed, in activated PBMC, the production and mRNA expression of interleukin-2 (IL-2), IL-4, tumor necrosis factor-α, and interferon-γ in a concentration-dependent manner.
This inhibition was not related to cell viability.
While 4-HM did not affect ERK phosphorylation and its downstream c-fos gene expression in PBMC induced by PHA, it decreased both NF-AT and NF-κB activation.
The upstream signaling of NF-AT and NF-κB, intracellular calcium concentrations ([Ca2+]i), and protein kinase C theta (PKC θ) activation in PHA-treated PBMC were reduced by 4-HM.
The data demonstrated that the suppressant effects of 4-HM on cell proliferation in PBMC activated by PHA appeared to be mediated, at least in part, through inhibition of Ca2+ mobilization and PKC θ activation, NF-AT and NF-κB activation, and cytokine transcripts and productions of PBMC.
We suggested that A.
blazei contained a potential immunomodulator 4-HM.
American Psychological Association (APA)
Tsai, Wei-Jern& Yang, Shih-Chien& Huang, Yu-Ling& Chen, Chien-Chih& Chuang, Kai-An& Kuo, Yuh-Chi. 2013. 4-Hydroxy-17-methylincisterol from Agaricus blazei Decreased Cytokine Production and Cell Proliferation in Human Peripheral Blood Mononuclear Cells via Inhibition of NF-AT and NF-κB Activation. Evidence-Based Complementary and Alternative Medicine،Vol. 2013, no. 2013, pp.1-14.
https://search.emarefa.net/detail/BIM-472074
Modern Language Association (MLA)
Tsai, Wei-Jern…[et al.]. 4-Hydroxy-17-methylincisterol from Agaricus blazei Decreased Cytokine Production and Cell Proliferation in Human Peripheral Blood Mononuclear Cells via Inhibition of NF-AT and NF-κB Activation. Evidence-Based Complementary and Alternative Medicine No. 2013 (2013), pp.1-14.
https://search.emarefa.net/detail/BIM-472074
American Medical Association (AMA)
Tsai, Wei-Jern& Yang, Shih-Chien& Huang, Yu-Ling& Chen, Chien-Chih& Chuang, Kai-An& Kuo, Yuh-Chi. 4-Hydroxy-17-methylincisterol from Agaricus blazei Decreased Cytokine Production and Cell Proliferation in Human Peripheral Blood Mononuclear Cells via Inhibition of NF-AT and NF-κB Activation. Evidence-Based Complementary and Alternative Medicine. 2013. Vol. 2013, no. 2013, pp.1-14.
https://search.emarefa.net/detail/BIM-472074
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-472074