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Transposon Invasion of the Paramecium Germline Genome Countered by a Domesticated PiggyBac Transposase and the NHEJ Pathway
Joint Authors
Dubois, Emeline
Régnier, Vinciane
Marmignon, Antoine
Bischerour, Julien
Bétermier, Mireille
Mathy, Nathalie
Source
International Journal of Evolutionary Biology
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-07-22
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Sequences related to transposons constitute a large fraction of extant genomes, but insertions within coding sequences have generally not been tolerated during evolution.
Thanks to their unique nuclear dimorphism and to their original mechanism of programmed DNA elimination from their somatic nucleus (macronucleus), ciliates are emerging model organisms for the study of the impact of transposable elements on genomes.
The germline genome of the ciliate Paramecium, located in its micronucleus, contains thousands of short intervening sequences, the IESs, which interrupt 47% of genes.
Recent data provided support to the hypothesis that an evolutionary link exists between Paramecium IESs and Tc1/mariner transposons.
During development of the macronucleus, IESs are excised precisely thanks to the coordinated action of PiggyMac, a domesticated piggyBac transposase, and of the NHEJ double-strand break repair pathway.
A PiggyMac homolog is also required for developmentally programmed DNA elimination in another ciliate, Tetrahymena.
Here, we present an overview of the life cycle of these unicellular eukaryotes and of the developmentally programmed genome rearrangements that take place at each sexual cycle.
We discuss how ancient domestication of a piggyBac transposase might have allowed Tc1/mariner elements to spread throughout the germline genome of Paramecium, without strong counterselection against insertion within genes.
American Psychological Association (APA)
Dubois, Emeline& Bischerour, Julien& Marmignon, Antoine& Mathy, Nathalie& Régnier, Vinciane& Bétermier, Mireille. 2012. Transposon Invasion of the Paramecium Germline Genome Countered by a Domesticated PiggyBac Transposase and the NHEJ Pathway. International Journal of Evolutionary Biology،Vol. 2012, no. 2012, pp.1-13.
https://search.emarefa.net/detail/BIM-472111
Modern Language Association (MLA)
Dubois, Emeline…[et al.]. Transposon Invasion of the Paramecium Germline Genome Countered by a Domesticated PiggyBac Transposase and the NHEJ Pathway. International Journal of Evolutionary Biology No. 2012 (2012), pp.1-13.
https://search.emarefa.net/detail/BIM-472111
American Medical Association (AMA)
Dubois, Emeline& Bischerour, Julien& Marmignon, Antoine& Mathy, Nathalie& Régnier, Vinciane& Bétermier, Mireille. Transposon Invasion of the Paramecium Germline Genome Countered by a Domesticated PiggyBac Transposase and the NHEJ Pathway. International Journal of Evolutionary Biology. 2012. Vol. 2012, no. 2012, pp.1-13.
https://search.emarefa.net/detail/BIM-472111
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-472111