![](/images/graphics-bg.png)
High-Performance Liquid Chromatography Assay for Moxifloxacin in Brain Tissue and Plasma : Validation in a Pharmacokinetic Study in a Murine Model of Cerebral Listeriosis
Joint Authors
Dubouch, Sophie
Lott, Marie-Catherine
Respaud, Renaud
Singlas, Eric
Le Monnier, Alban
Grayo, Solene
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-03-14
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Moxifloxacin is a broad-spectrum antibacterial 8-methoxy-fluoroquinolone.
In order to evaluate the pharmacokinetic properties of moxifloxacin in mouse plasma and brain tissue, we developed a high-performance liquid chromatography (HPLC) method.
This study was based on single-drug delivery, intravenously dosed in a central listeriosis murine model.
The method employed a reversed-phase Lichrospher RP-18 with a precolumn (250×4.6 mm) and a mobile phase composed of a mixture of acetonitrile, methanol, and citric buffer (pH=3.5) with sodium dodecyl sulfate and tetrabutylammonium bromide.
Fluorescence detection was performed at an excitation wavelength of 290 nm and an emission wavelength of 550 nm.
The relative standard deviation of intra- and inter-day assays was <10%.
This validated method led to a short retention time (8.0 min) for moxifloxacin.
The standard curves were linear from 5–250 μg/L in plasma and from 0.1–2.5 μg/g of brain tissue.
The limits of quantification were 5 μg/L in plasma and 0.1 μg/g in brain tissue.
The method enabled the detection of systemic antimicrobial in plasma and in CNS in Listeria-infected mice.
Injected moxifloxacin passed through the encephalic barrier within a 30 to 60 min after injection time frame.
Moxifloxacin pharmacokinetics are modeled in an infected model compared to control mice.
American Psychological Association (APA)
Respaud, Renaud& Grayo, Solene& Singlas, Eric& Dubouch, Sophie& Le Monnier, Alban& Lott, Marie-Catherine. 2012. High-Performance Liquid Chromatography Assay for Moxifloxacin in Brain Tissue and Plasma : Validation in a Pharmacokinetic Study in a Murine Model of Cerebral Listeriosis. Journal of Analytical Methods in Chemistry،Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-472136
Modern Language Association (MLA)
Respaud, Renaud…[et al.]. High-Performance Liquid Chromatography Assay for Moxifloxacin in Brain Tissue and Plasma : Validation in a Pharmacokinetic Study in a Murine Model of Cerebral Listeriosis. Journal of Analytical Methods in Chemistry No. 2012 (2012), pp.1-7.
https://search.emarefa.net/detail/BIM-472136
American Medical Association (AMA)
Respaud, Renaud& Grayo, Solene& Singlas, Eric& Dubouch, Sophie& Le Monnier, Alban& Lott, Marie-Catherine. High-Performance Liquid Chromatography Assay for Moxifloxacin in Brain Tissue and Plasma : Validation in a Pharmacokinetic Study in a Murine Model of Cerebral Listeriosis. Journal of Analytical Methods in Chemistry. 2012. Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-472136
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-472136