Stimulation of MMP-1 and CCL2 by NAMPT in PDL Cells
Joint Authors
Jepsen, Søren
Jäger, Andreas
Deschner, James
Cirelli, Joni Augusto
Nokhbehsaim, Marjan
Eick, Sigrun
Hoffmann, Per
Nogueira, Andressa Vilas Boas
Herms, Stefan
Fröhlich, Holger
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-08-22
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Periodontitis is an inflammatory disease caused by pathogenic microorganisms and characterized by the destruction of the periodontium.
Obese individuals have an increased risk of periodontitis, and elevated circulating levels of adipokines, such as nicotinamide phosphoribosyltransferase (NAMPT), may be a pathomechanistic link between both diseases.
The aim of this in vitro study was to examine the regulation of periodontal ligament (PDL) cells by NAMPT and its production under inflammatory and infectious conditions.
NAMPT caused a significant upregulation of 9 genes and downregulation of 3 genes, as analyzed by microarray analysis.
Eight of these genes could be confirmed by real-time PCR: NAMPT induced a significant upregulation of EGR1, MMP-1, SYT7, ITPKA, CCL2, NTM, IGF2BP3, and NRP1.
NAMPT also increased significantly the MMP-1 and CCL2 protein synthesis.
NAMPT was significantly induced by interleukin-1β and the periodontal microorganism P.
gingivalis.
NAMPT may contribute to periodontitis through upregulation of MMP-1 and CCL2 in PDL cells.
Increased NAMPT levels, as found in obesity, may therefore represent a mechanism whereby obesity could confer an increased risk of periodontitis.
Furthermore, microbial and inflammatory signals may enhance the NAMPT synthesis in PDL cells and thereby contribute to the increased gingival and serum levels of this adipokine, as found in periodontitis.
American Psychological Association (APA)
Nokhbehsaim, Marjan& Eick, Sigrun& Nogueira, Andressa Vilas Boas& Hoffmann, Per& Herms, Stefan& Fröhlich, Holger…[et al.]. 2013. Stimulation of MMP-1 and CCL2 by NAMPT in PDL Cells. Mediators of Inflammation،Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-472220
Modern Language Association (MLA)
Nokhbehsaim, Marjan…[et al.]. Stimulation of MMP-1 and CCL2 by NAMPT in PDL Cells. Mediators of Inflammation No. 2013 (2013), pp.1-12.
https://search.emarefa.net/detail/BIM-472220
American Medical Association (AMA)
Nokhbehsaim, Marjan& Eick, Sigrun& Nogueira, Andressa Vilas Boas& Hoffmann, Per& Herms, Stefan& Fröhlich, Holger…[et al.]. Stimulation of MMP-1 and CCL2 by NAMPT in PDL Cells. Mediators of Inflammation. 2013. Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-472220
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-472220