The 3-D Culture and In Vivo Growth of the Human Hepatocellular Carcinoma Cell Line HepG2 in a Self-Assembling Peptide Nanofiber Scaffold
Joint Authors
Liu, Yanfei
Liu, Bo
Zhao, Xiaojun
Yang, Zehong
Wu, Min
Source
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-09-02
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Engineering Sciences and Information Technology
Chemistry
Civil Engineering
Abstract EN
We report the use of the RADA16-I scaffold to mimic the ECM microenvironment and support tumor cell adherence and survival.
Cellular morphology, proliferation, adhesion ability, and in vivo tumor formation were studied in the human hepatocellular carcinoma cell line HepG2 in the 3-D RADA16-I scaffold.
No significant differences in HepG2 cell proliferation, adhesion, and albumin secretion were observed in the peptide scaffold compared to collagen I.
Furthermore, the HepG2 cells precultured in the peptide scaffold showed a higher proliferation rate and formed significantly bigger tumors when compared to cells grown on a traditional 2D monolayer, suggesting that the 3-D RADA16-I scaffold can mimic the tumor microenvironment and promote a malignant phenotype in HepG2 cells.
Our results indicate that the RADA16-I scaffold can serve as an ideal model for tumorigenesis, growth, local invasion, and metastasis.
American Psychological Association (APA)
Wu, Min& Yang, Zehong& Liu, Yanfei& Liu, Bo& Zhao, Xiaojun. 2010. The 3-D Culture and In Vivo Growth of the Human Hepatocellular Carcinoma Cell Line HepG2 in a Self-Assembling Peptide Nanofiber Scaffold. Journal of Nanomaterials،Vol. 2010, no. 2010, pp.1-7.
https://search.emarefa.net/detail/BIM-472233
Modern Language Association (MLA)
Wu, Min…[et al.]. The 3-D Culture and In Vivo Growth of the Human Hepatocellular Carcinoma Cell Line HepG2 in a Self-Assembling Peptide Nanofiber Scaffold. Journal of Nanomaterials No. 2010 (2010), pp.1-7.
https://search.emarefa.net/detail/BIM-472233
American Medical Association (AMA)
Wu, Min& Yang, Zehong& Liu, Yanfei& Liu, Bo& Zhao, Xiaojun. The 3-D Culture and In Vivo Growth of the Human Hepatocellular Carcinoma Cell Line HepG2 in a Self-Assembling Peptide Nanofiber Scaffold. Journal of Nanomaterials. 2010. Vol. 2010, no. 2010, pp.1-7.
https://search.emarefa.net/detail/BIM-472233
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-472233