Quantification of Lumefantrine in Human Plasma Using LC-MSMS and Its Application to a Bioequivalence Study
Joint Authors
Mangaonkar, Kiran V.
Pingale, Satish G.
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-09-23
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Pharmacy, Health & Medical Sciences
Economics & Business Administration
Pharmacology
Abstract EN
An analytical method based on protein precipitation has been developed and validated for analysis of lumefantrine in human plasma.
Artesunate was used as an internal standard for lumefantrine.
Inertsil ODS column provided chromatographic separation of analytes followed by detection with mass spectrometry.
The method involves simple isocratic chromatographic condition and mass spectrometric detection in the positive ionization mode using an API-3000 system.
The total run time was 2.5 minutes.
The proposed method has been validated with linear range of 200–20000 ng/mL for lumefantrine.
The intrarun and interrun precision values are within 6.66% and 5.56%, respectively, for lumefantrine at the lower limit of quantification level.
The overall recovery for lumefantrine and artesunate was 93.16% and 91.05%, respectively.
This validated method was used successfully for analysis of plasma samples from a bioequivalence study.
American Psychological Association (APA)
Pingale, Satish G.& Mangaonkar, Kiran V.. 2012. Quantification of Lumefantrine in Human Plasma Using LC-MSMS and Its Application to a Bioequivalence Study. Journal of Pharmaceutics،Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-472293
Modern Language Association (MLA)
Pingale, Satish G.& Mangaonkar, Kiran V.. Quantification of Lumefantrine in Human Plasma Using LC-MSMS and Its Application to a Bioequivalence Study. Journal of Pharmaceutics No. 2013 (2013), pp.1-8.
https://search.emarefa.net/detail/BIM-472293
American Medical Association (AMA)
Pingale, Satish G.& Mangaonkar, Kiran V.. Quantification of Lumefantrine in Human Plasma Using LC-MSMS and Its Application to a Bioequivalence Study. Journal of Pharmaceutics. 2012. Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-472293
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-472293