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Early Steps in the DNA Base Excision Repair Pathway of a Fission Yeast Schizosaccharomyces pombe
Joint Authors
Kanamitsu, Kyoichiro
Ikeda, Shogo
Source
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-09-16
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
DNA base excision repair (BER) accounts for maintaining genomic integrity by removing damaged bases that are generated endogenously or induced by genotoxic agents.
In this paper, we describe the roles of enzymes functioning in the early steps of BER in fission yeast.
Although BER is an evolutionarily conserved process, some unique features of the yeast repair pathway were revealed by genetic and biochemical approaches.
AP sites generated by monofunctional DNA glycosylases are incised mainly by AP lyase activity of Nth1p, a sole bifunctional glycosylase in yeast, to leave a blocked 3′ end.
The major AP endonuclease Apn2p functions predominantly in removing the 3′ block.
Finally, a DNA polymerase fills the gap, and a DNA ligase seals the nick (Nth1p-dependent or short patch BER).
Apn1p backs up Apn2p.
In long patch BER, Rad2p endonuclease removes flap DNA containing a lesion after DNA synthesis.
A UV-specific endonuclease Uve1p engages in an alternative pathway by nicking DNA on the 5′ side of oxidative damage.
Nucleotide excision repair and homologous recombination are involved in repair of BER intermediates including the AP site and single-strand break with the 3′ block.
Other enzymes working in 3′ end processing are also discussed.
American Psychological Association (APA)
Kanamitsu, Kyoichiro& Ikeda, Shogo. 2010. Early Steps in the DNA Base Excision Repair Pathway of a Fission Yeast Schizosaccharomyces pombe. Journal of Nucleic Acids،Vol. 2010, no. 2010, pp.1-9.
https://search.emarefa.net/detail/BIM-472584
Modern Language Association (MLA)
Kanamitsu, Kyoichiro& Ikeda, Shogo. Early Steps in the DNA Base Excision Repair Pathway of a Fission Yeast Schizosaccharomyces pombe. Journal of Nucleic Acids No. 2010 (2010), pp.1-9.
https://search.emarefa.net/detail/BIM-472584
American Medical Association (AMA)
Kanamitsu, Kyoichiro& Ikeda, Shogo. Early Steps in the DNA Base Excision Repair Pathway of a Fission Yeast Schizosaccharomyces pombe. Journal of Nucleic Acids. 2010. Vol. 2010, no. 2010, pp.1-9.
https://search.emarefa.net/detail/BIM-472584
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-472584