Differential Expression of Osteo-Modulatory Molecules in Periodontal Ligament Stem Cells in Response to Modified Titanium Surfaces
Joint Authors
Kwon, Yong-Dae
Kim, So Yeon
Heo, Jung Sun
Lee, Jung-Woo
Yoo, Ji-Yeon
Ohe, Joo-Young
Moon, Ji-Hoi
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-06-25
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
This study assessed differential gene expression of signaling molecules involved in osteogenic differentiation of periodontal ligament stem cells (PDLSCs) subjected to different titanium (Ti) surface types.
PDLSCs were cultured on tissue culture polystyrene (TCPS), and four types of Ti discs (PT, SLA, hydrophilic PT (pmodPT), and hydrophilic SLA (modSLA)) with no osteoinductive factor and then osteogenic activity, including alkaline phosphatase (ALP) activity, mRNA expression of runt-related gene 2, osterix, FOSB, FRA1, and protein levels of osteopontin and collagen type IA, were examined.
The highest osteogenic activity appeared in PDLSCs cultured on SLA, compared with the TCPS and other Ti surfaces.
The role of surface properties in affecting signaling molecules to modulate PDLSC behavior was determined by examining the regulation of Wnt pathways.
mRNA expression of the canonical Wnt signaling molecules, Wnt3a and β-catenin, was higher on SLA and modSLA than on smooth surfaces, but gene expression of the calcium-dependent Wnt signaling molecules Wnt5a, calmodulin, and NFATc1 was increased significantly on PT and pmodPT.
Moreover, integrin α2/β1, sonic hedgehog, and Notch signaling molecules were affected differently by each surface modification.
In conclusion, surface roughness and hydrophilicity can affect differential Wnt pathways and signaling molecules, targeting the osteogenic differentiation of PDLSCs.
American Psychological Association (APA)
Kim, So Yeon& Yoo, Ji-Yeon& Ohe, Joo-Young& Lee, Jung-Woo& Moon, Ji-Hoi& Kwon, Yong-Dae…[et al.]. 2014. Differential Expression of Osteo-Modulatory Molecules in Periodontal Ligament Stem Cells in Response to Modified Titanium Surfaces. BioMed Research International،Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-472689
Modern Language Association (MLA)
Kim, So Yeon…[et al.]. Differential Expression of Osteo-Modulatory Molecules in Periodontal Ligament Stem Cells in Response to Modified Titanium Surfaces. BioMed Research International No. 2014 (2014), pp.1-12.
https://search.emarefa.net/detail/BIM-472689
American Medical Association (AMA)
Kim, So Yeon& Yoo, Ji-Yeon& Ohe, Joo-Young& Lee, Jung-Woo& Moon, Ji-Hoi& Kwon, Yong-Dae…[et al.]. Differential Expression of Osteo-Modulatory Molecules in Periodontal Ligament Stem Cells in Response to Modified Titanium Surfaces. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-472689
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-472689