Biochemical Characterization and 16S rRNA Sequencing of Few Lipase-Producing Thermophilic Bacteria from Taptapani Hot Water Spring, Orissa, India
Joint Authors
Bisht, Satpal S.
Panda, Amrita K.
Source
Biotechnology Research International
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-04-14
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Information Technology and Computer Science
Abstract EN
Three lipase-producing thermophilic bacteria (AK-P1, AK-P2, and AK-P3) were isolated from the Taptapani hot water spring in Orissa, India.
The crude extra cellular lipases from cell-free culture supernatant were reacted in an olive oil mixture, and their lipolytic activities were compared.
Identification of the bacteria was carried out using biochemical tests, 16SrRNA sequencing and sequences submitted to NCBI GenBank.
Strain AK-P3, exhibited the highest lipolytic activity of 5.5 U/mL was identified as Porphyrobacter sp.
The lipolytic activities of strains AK-P1 and AK-P 2 were 4.5 U/mL and 3.5 U/mL, respectively.
Strains AK-P1 and AK-P2 were identified as Acinetobacter sp.
and Brevibacillus spp.
The GenBank accession numbers of the 16S rRNA gene sequences determined in this study for the strains AK-P1, AK-P2, and AK-P3 are HM359120, HM359119, and HM359118, respectively.
American Psychological Association (APA)
Bisht, Satpal S.& Panda, Amrita K.. 2011. Biochemical Characterization and 16S rRNA Sequencing of Few Lipase-Producing Thermophilic Bacteria from Taptapani Hot Water Spring, Orissa, India. Biotechnology Research International،Vol. 2011, no. 2011, pp.1-5.
https://search.emarefa.net/detail/BIM-472737
Modern Language Association (MLA)
Bisht, Satpal S.& Panda, Amrita K.. Biochemical Characterization and 16S rRNA Sequencing of Few Lipase-Producing Thermophilic Bacteria from Taptapani Hot Water Spring, Orissa, India. Biotechnology Research International No. 2011 (2011), pp.1-5.
https://search.emarefa.net/detail/BIM-472737
American Medical Association (AMA)
Bisht, Satpal S.& Panda, Amrita K.. Biochemical Characterization and 16S rRNA Sequencing of Few Lipase-Producing Thermophilic Bacteria from Taptapani Hot Water Spring, Orissa, India. Biotechnology Research International. 2011. Vol. 2011, no. 2011, pp.1-5.
https://search.emarefa.net/detail/BIM-472737
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-472737
