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Mechanisms Underlying the Antiproliferative and Prodifferentiative Effects of Psoralen on Adult Neural Stem Cells via DNA Microarray
Joint Authors
Dong, Jing-cheng
Chen, Yang
Ning, You
Huang, Jian-Hua
Zhang, Xin-Min
Shen, Zi-Yin
Bian, Qin
Xia, Shi-Jin
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-07-30
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Abstract EN
Adult neural stem cells (NSCs) persist throughout life to replace mature cells that are lost during turnover, disease, or injury.
The investigation of NSC creates novel treatments for central nervous system (CNS) injuries and neurodegenerative disorders.
The plasticity and reparative potential of NSC are regulated by different factors, which are critical for neurological regenerative medicine research.
We investigated the effects of Psoralen, which is the mature fruit of Psoralea corylifolia L., on NSC behaviors and the underlying mechanisms.
The self-renewal and proliferation of NSC were examined.
We detected neuron- and/or astrocyte-specific markers using immunofluorescence and Western blotting, which could evaluate NSC differentiation.
Psoralen treatment significantly inhibited neurosphere formation in a dose-dependent manner.
Psoralen treatment increased the expression of the astrocyte-specific marker but decreased neuron-specific marker expression.
These results suggested that Psoralen was a differentiation inducer in astrocyte.
Differential gene expression following Psoralen treatment was screened using DNA microarray and confirmed by quantitative real-time PCR.
Our microarray study demonstrated that Psoralen could effectively regulate the specific gene expression profile of NSC.
The genes involved in the classification of cellular differentiation, proliferation, and metabolism, the transcription factors belonging to Ets family, and the hedgehog pathway may be closely related to the regulation.
American Psychological Association (APA)
Ning, You& Huang, Jian-Hua& Xia, Shi-Jin& Bian, Qin& Chen, Yang& Zhang, Xin-Min…[et al.]. 2013. Mechanisms Underlying the Antiproliferative and Prodifferentiative Effects of Psoralen on Adult Neural Stem Cells via DNA Microarray. Evidence-Based Complementary and Alternative Medicine،Vol. 2013, no. 2013, pp.1-15.
https://search.emarefa.net/detail/BIM-472758
Modern Language Association (MLA)
Ning, You…[et al.]. Mechanisms Underlying the Antiproliferative and Prodifferentiative Effects of Psoralen on Adult Neural Stem Cells via DNA Microarray. Evidence-Based Complementary and Alternative Medicine No. 2013 (2013), pp.1-15.
https://search.emarefa.net/detail/BIM-472758
American Medical Association (AMA)
Ning, You& Huang, Jian-Hua& Xia, Shi-Jin& Bian, Qin& Chen, Yang& Zhang, Xin-Min…[et al.]. Mechanisms Underlying the Antiproliferative and Prodifferentiative Effects of Psoralen on Adult Neural Stem Cells via DNA Microarray. Evidence-Based Complementary and Alternative Medicine. 2013. Vol. 2013, no. 2013, pp.1-15.
https://search.emarefa.net/detail/BIM-472758
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-472758