Low-Power Ultrasounds as a Tool to Culture Human Osteoblasts inside Cancellous Hydroxyapatite
Joint Authors
Benazzo, Francesco
Saino, Enrica
Cusella De Angelis, Maria Gabriella
Visai, Livia
Magenes, Giovanni
Fassina, Lorenzo
Source
Bioinorganic Chemistry and Applications
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-03-31
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Bone graft substitutes and cancellous biomaterials have been widely used to heal critical-size long bone defects due to trauma, tumor resection, and tissue degeneration.
In particular, porous hydroxyapatite is widely used in reconstructive bone surgery owing to its biocompatibility.
In addition, the in vitro modification of cancellous hydroxyapatite with osteogenic signals enhances the tissue regeneration in vivo, suggesting that the biomaterial modification could play an important role in tissue engineering.
In this study, we have followed a tissue-engineering strategy where ultrasonically stimulated SAOS-2 human osteoblasts proliferated and built their extracellular matrix inside a porous hydroxyapatite scaffold.
The ultrasonic stimulus had the following parameters: average power equal to 149 mW and frequency of 1.5 MHz.
In comparison with control conditions, the ultrasonic stimulus increased the cell proliferation and the surface coating with bone proteins (decorin, osteocalcin, osteopontin, type-I collagen, and type-III collagen).
The mechanical stimulus aimed at obtaining a better modification of the biomaterial internal surface in terms of cell colonization and coating with bone matrix.
The modified biomaterial could be used, in clinical applications, as an implant for bone repair.
American Psychological Association (APA)
Fassina, Lorenzo& Saino, Enrica& Cusella De Angelis, Maria Gabriella& Magenes, Giovanni& Benazzo, Francesco& Visai, Livia. 2010. Low-Power Ultrasounds as a Tool to Culture Human Osteoblasts inside Cancellous Hydroxyapatite. Bioinorganic Chemistry and Applications،Vol. 2010, no. 2010, pp.1-8.
https://search.emarefa.net/detail/BIM-472930
Modern Language Association (MLA)
Fassina, Lorenzo…[et al.]. Low-Power Ultrasounds as a Tool to Culture Human Osteoblasts inside Cancellous Hydroxyapatite. Bioinorganic Chemistry and Applications No. 2010 (2010), pp.1-8.
https://search.emarefa.net/detail/BIM-472930
American Medical Association (AMA)
Fassina, Lorenzo& Saino, Enrica& Cusella De Angelis, Maria Gabriella& Magenes, Giovanni& Benazzo, Francesco& Visai, Livia. Low-Power Ultrasounds as a Tool to Culture Human Osteoblasts inside Cancellous Hydroxyapatite. Bioinorganic Chemistry and Applications. 2010. Vol. 2010, no. 2010, pp.1-8.
https://search.emarefa.net/detail/BIM-472930
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-472930