PH1 : An Archaeovirus of Haloarcula hispanica Related to SH1 and HHIV-2
Joint Authors
Chen, Chung-Pin
Tang, Sen-Lin
Porter, Kate
Chiang, Pei-Wen
Dyall-Smith, Mike
Hong, Mei-Jhu
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-17, 17 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-03-21
Country of Publication
Egypt
No. of Pages
17
Main Subjects
Abstract EN
Halovirus PH1 infects Haloarcula hispanica and was isolated from an Australian salt lake.
The burst size in single-step growth conditions was 50–100 PFU/cell, but cell density did not decrease until well after the rise (4–6 hr p.i.), indicating that the virus could exit without cell lysis.
Virions were round, 51 nm in diameter, displayed a layered capsid structure, and were sensitive to chloroform and lowered salt concentration.
The genome is linear dsDNA, 28,064 bp in length, with 337 bp terminal repeats and terminal proteins, and could transfect haloarchaeal species belonging to five different genera.
The genome is predicted to carry 49 ORFs, including those for structural proteins, several of which were identified by mass spectroscopy.
The close similarity of PH1 to SH1 (74% nucleotide identity) allowed a detailed description and analysis of the differences (divergent regions) between the two genomes, including the detection of repeat-mediated deletions.
The relationship of SH1-like and pleolipoviruses to previously described genomic loci of virus and plasmid-related elements (ViPREs) of haloarchaea revealed an extensive level of recombination between the known haloviruses.
PH1 is a member of the same virus group as SH1 and HHIV-2, and we propose the name halosphaerovirus to accommodate these viruses.
American Psychological Association (APA)
Porter, Kate& Tang, Sen-Lin& Chen, Chung-Pin& Chiang, Pei-Wen& Hong, Mei-Jhu& Dyall-Smith, Mike. 2013. PH1 : An Archaeovirus of Haloarcula hispanica Related to SH1 and HHIV-2. Archaea،Vol. 2013, no. 2013, pp.1-17.
https://search.emarefa.net/detail/BIM-472934
Modern Language Association (MLA)
Porter, Kate…[et al.]. PH1 : An Archaeovirus of Haloarcula hispanica Related to SH1 and HHIV-2. Archaea No. 2013 (2013), pp.1-17.
https://search.emarefa.net/detail/BIM-472934
American Medical Association (AMA)
Porter, Kate& Tang, Sen-Lin& Chen, Chung-Pin& Chiang, Pei-Wen& Hong, Mei-Jhu& Dyall-Smith, Mike. PH1 : An Archaeovirus of Haloarcula hispanica Related to SH1 and HHIV-2. Archaea. 2013. Vol. 2013, no. 2013, pp.1-17.
https://search.emarefa.net/detail/BIM-472934
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-472934