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An Inducible Expression System of the Calcium-Activated Potassium Channel 4 to Study the Differential Impact on Embryonic Stem Cells
Joint Authors
Weidgang, Clair
Ansorge, Daniel
Linta, Leonhard
Iacovino, Michelina
Liebau, Stefan
Kyba, Michael
von Wichert, Götz
Kleger, Alexander
Tischendorf, Michael
Stockmann, Marianne
Boeckers, Tobias M.
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-09-15
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Abstract EN
Rationale.
The family of calcium-activated potassium channels consists of four members with varying biological functions and conductances.
Besides membrane potential modulation, SK channels have been found to be involved in cardiac pacemaker cell development from ES cells and morphological shaping of neural stem cells.
Objective.
Distinct SK channel subtype expression in ES cells might elucidate their precise impact during cardiac development.
We chose SK channel subtype 4 as a potential candidate influencing embryonic stem cell differentiation.
Methods.
We generated a doxycycline inducible mouse ES cell line via targeted homologous recombination of a cassette expressing a bicistronic construct encoding SK4 and a fluorophore from the murine HPRT locus.
Conclusion.
We characterized the mouse ES cell line iSK4-AcGFP.
The cassette is readily expressed under the control of doxycycline, and the overexpression of SK4 led to an increase in cardiac and pacemaker cell differentiation thereby serving as a unique tool to characterize the cell biological variances due to specific SK channel overexpression.
American Psychological Association (APA)
Liebau, Stefan& Tischendorf, Michael& Ansorge, Daniel& Linta, Leonhard& Stockmann, Marianne& Weidgang, Clair…[et al.]. 2011. An Inducible Expression System of the Calcium-Activated Potassium Channel 4 to Study the Differential Impact on Embryonic Stem Cells. Stem Cells International،Vol. 2011, no. 2011, pp.1-12.
https://search.emarefa.net/detail/BIM-472975
Modern Language Association (MLA)
Liebau, Stefan…[et al.]. An Inducible Expression System of the Calcium-Activated Potassium Channel 4 to Study the Differential Impact on Embryonic Stem Cells. Stem Cells International No. 2011 (2011), pp.1-12.
https://search.emarefa.net/detail/BIM-472975
American Medical Association (AMA)
Liebau, Stefan& Tischendorf, Michael& Ansorge, Daniel& Linta, Leonhard& Stockmann, Marianne& Weidgang, Clair…[et al.]. An Inducible Expression System of the Calcium-Activated Potassium Channel 4 to Study the Differential Impact on Embryonic Stem Cells. Stem Cells International. 2011. Vol. 2011, no. 2011, pp.1-12.
https://search.emarefa.net/detail/BIM-472975
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-472975