Construction and Characterization of a Bacterial Artificial Chromosome Library for the A-Genome of Cotton (G. arboreum L.)
Joint Authors
Hu, Yan
Zhang, Tianzhen
Lu, Yamin
Guo, Wangzhen
Ma, Dan
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-08-23
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
A bacterial artificial chromosome (BAC) library for the A-genome of cotton has been constructed from the leaves of G.
arboreum L cv.
Jianglinzhongmian.
It is used as elite A-genome germplasm resources in the present cotton breeding program and has been used to build a genetic reference map of cotton.
The BAC library consists of 123,648 clones stored in 322 384-well plates.
Statistical analysis of a set of 103 randomly selected BAC clones indicated that each clone has an average insert length of 100.2 kb per plasmid, with a range of 30 to 190 kb.
Theoretically, this represents 7.2 haploid genome equivalents based on an A-genome size of 1697 Mb.
The BAC library has been arranged in column pools and superpools allowing screening with various PCR-based markers.
In the future, the A-genome cotton BAC library will serve as both a giant gene resource and a valuable tool for map-based gene isolation, physical mapping and comparative genome analysis.
American Psychological Association (APA)
Hu, Yan& Lu, Yamin& Ma, Dan& Guo, Wangzhen& Zhang, Tianzhen. 2010. Construction and Characterization of a Bacterial Artificial Chromosome Library for the A-Genome of Cotton (G. arboreum L.). Journal of Biomedicine and Biotechnology،Vol. 2011, no. 2011, pp.1-5.
https://search.emarefa.net/detail/BIM-473010
Modern Language Association (MLA)
Hu, Yan…[et al.]. Construction and Characterization of a Bacterial Artificial Chromosome Library for the A-Genome of Cotton (G. arboreum L.). Journal of Biomedicine and Biotechnology No. 2011 (2011), pp.1-5.
https://search.emarefa.net/detail/BIM-473010
American Medical Association (AMA)
Hu, Yan& Lu, Yamin& Ma, Dan& Guo, Wangzhen& Zhang, Tianzhen. Construction and Characterization of a Bacterial Artificial Chromosome Library for the A-Genome of Cotton (G. arboreum L.). Journal of Biomedicine and Biotechnology. 2010. Vol. 2011, no. 2011, pp.1-5.
https://search.emarefa.net/detail/BIM-473010
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-473010